文献类型：期刊文献

英文题名：Electrochemical detection of DNA hybridization by a zirconia modified diamond electrode

作者：Liu, Baixiang[2];Hu, Jingping[1];Foord, John S.[1]

机构：[1]Univ Oxford, Dept Chem, Chem Res Lab, Oxford OX1 3TA, England;[2]E China Univ Sci & Technol, Dept Phys, Shanghai 200237, Peoples R China

年份：2012

卷号：19

期号：1

起止页码：46

外文期刊名：ELECTROCHEMISTRY COMMUNICATIONS

收录：;EI(收录号：20122315088151);WOS:【SCI-EXPANDED(收录号:WOS:000305767700013)】；

基金：We would like to acknowledge the financial support from EPSRC with grant no. EP/F025513/1. Baixiang acknowledges the financial support of the China Scholarship Council.

语种：英文

外文关键词：DNA; Diamond; Zirconia; Bio-sensor; Methylene blue

摘要：The use of a zirconia thin layer coating to attach phosphate terminated DNA oligonucleotides to a diamond electrode was explored. This DNA functionalised electrode shows an enhanced redox response to the redox mediator methylene blue, which is quenched in the presence of complementary DNA oligonucleotides, enabling the highly sensitive and selective detection of such species in solution. The study shows that the electrodeposition of zirconia on diamond electrodes can be used as an effective and easily implemented route for surface functionalisation and biosensor fabrication using this electrode material. (C) 2012 Elsevier B.V. All rights reserved.