文献类型：期刊文献

英文题名：Dominance of Giardia duodenalis assemblage A and Enterocytozoon bieneusi genotype BEB6 in sheep in Inner Mongolia, China

作者：Ye, Jianbin[1];Xiao, Lihua[2];Wang, Yuanfei[1];Guo, Yaqiong[1,2];Roellig, Dawn M.[2];Feng, Yaoyu[1]

机构：[1]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Ctr Dis Control & Prevent, Atlanta, GA 30329 USA

年份：2015

卷号：210

期号：3-4

起止页码：235

外文期刊名：VETERINARY PARASITOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000356553700014)】；

基金：This work was supported by the National Natural Science Foundation of China (31229005 and 31110103901), National Special Fund for State Key Laboratory of Bioreactor Engineering, China (No. 2060204), Fundamental Research Funds for the Central Universities, China, and Centers for Disease Control and Prevention.

语种：英文

外文关键词：Giardia duodenalis; Enterocytozoon bieneusi; Sheep; Zoonosis; Genotype; Molecular epidemiology

摘要：To examine the occurrence and genotype distribution of Giardia duodenalis and Enterocytozoon bieneusi in sheep, fecal specimens were collected from 162 lambs and 213 ewes on seven farms in the Hulunbeier Prairie in Inner Mongolia, China. By PCR analysis of the triosephosphate isomerase gene, 16 of the 375 (4.3%) specimens were positive for G. duodenalis, with 13 sequenced successfully belonging to assemblage A. Lambs had a significantly higher infection rate than ewes (8.6% versus 0.9%, respectively). The dominance of assemblage A in sheep was supported by PCR analysis of the beta-giardin and glutamate dehydrogenase genes; 4 of 7 beta-giardin-positive specimens and 3 of 5 glutamate dehydrogenase-positive specimens were identified as assemblage A. A much higher occurrence of E. bieneusi was detected by PCR analysis of the ribosomal internal transcribed spacer; 77.8% of lambs and 62.9% of ewes were positive for the pathogen. Two genotypes of E. bieneusi were found: BEB6 and CM7. Genotype BEB6 was seen in 237 animals and on all seven farms, whereas genotype CM7 was detected in 23 animals from six farms. These data indicate that sheep in Inner Mongolia are commonly infected with G. duodenalis assemblage A and E. bieneusi genotype BEB6, two zoonotic pathogens in China. (C) 2015 Elsevier B.V. All rights reserved.