文献类型：期刊文献

中文题名：大肠杆菌发酵产L-色氨酸培养基及补料优化

英文题名：Optimization of Medium and Feeding Process for L-Tryptophan Production Fermented by Escherichia coli

作者：朱晓雯[1];王泽建[1];黎亮[1];吴杰群[2];庄英萍[1]

机构：[1]华东理工大学,生物反应器工程国家重点实验室,国家生化工程技术研究中心,上海200237;[2]浙江工业大学生物工程学院,浙江杭州310014

年份：2020

卷号：41

期号：2

起止页码：146

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2017】；

语种：中文

中文关键词：大肠杆菌;L-色氨酸;发酵;培养基优化;补料工艺

外文关键词：Escherichia coli;L-tryptophan;fermentation;medium optimization;feeding process

摘要：为了进一步提高优化大肠杆菌发酵产L-色氨酸的产量,采用响应面法优化了原初始发酵培养基组合成分,建立了乙酸铵-玉米浆补料模式。结果表明优化后培养基组合:0.12%硫酸铵、0.7%磷酸二氢钾、0.15%一水柠檬酸、0.28%七水硫酸镁、0.05%酵母粉、0810 0.39%乙酸铵、0.3%玉米浆。5 L罐的培养验证表明,玉米浆和乙酸铵是影响L-色氨酸产量的主要成分因素,优化后L-色氨酸产量提高了35.4%,发酵结束达到24.53 g/L,单位菌体L-色氨酸产量提高了19.8%,达到0.272 g/OD,糖酸转化率提高了27%,为L-色氨酸发酵提供一定的参考。
In order to further increase the production of L-tryptophan fermented by Escherichia coli,response surface methodology design were used to optimize the composition of original fermentation medium. Based on the conditions,the feeding mode of ammonium acetate-corn syrup was established. The results showed that the combination of optimized medium were as follows:0.12% ammonium sulfate,0.7% potassium dihydrogen phosphate,0.15% citric acid monohydrate,0.28% magnesium sulfate heptahydrate,0.05% yeast powder 0810,0.39% ammonium acetate,0.3% corn syrup. The validation culture in 5 L tank showed that corn syrup and ammonium acetate were the main factors affecting the production of L-tryptophan. After optimization,the yield of L-tryptophan increased by 35.4%,reaching 24.53 g/L at the end of fermentation. The yield of L-tryptophan in per unit cell increased by 19.8%,reaching 0.272 g/OD. The conversion rate of glucose was increased by 27%.And it would provide some reference for L-tryptophan fermentation.