文献类型：期刊文献

英文题名：Metformin regulates cellulase production in Trichoderma reesei via calcium signaling and mitochondrial function

作者：Wang, Jiajia[1];Chen, Yumeng[1];Cong, Jiawei[1];Wang, Wei[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2024

卷号：23

期号：1

外文期刊名：MICROBIAL CELL FACTORIES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001360489300001)】；

基金：Not applicable.

语种：英文

外文关键词：Trichoderma reesei; Metformin; Cellulase; Calcium signaling; ROS; Mitochondria

摘要：BackgroundTrichoderma reesei is renowned for its cellulase-producing ability and is used for biofuel production from lignocellulose. In plants and fungi, cellulase production is induced by cellulose and suppressed by glucose; however, whether metformin can enhance cellulase production and mitochondrial function in T. reesei remains unclear. Metformin reduces blood glucose levels by inhibiting hepatic gluconeogenesis; therefore, it is worth investigating whether metformin transmission modulates cellulase biosynthesis in T. reesei.ResultsMetformin increased cellulase production and the transcription of cellulase-related genes. It also enhanced the concentrations of Ca2+ in the cytosol and mitochondria and regulated the transcription levels of cellulase-related genes by modulating calcium homeostasis in T. reesei QM6a. In addition, metformin was identified as an antioxidant that can enhance cellulase activity by reducing reactive oxygen species (ROS). Our results demonstrated that metformin influences the state of mitochondria by enhancing mitochondrial activity and membrane potential to promote cellulase production.ConclusionCollectively, these results indicate that metformin enhances cellulase production, scavenges ROS, and protects mitochondrial activity in T. reesei.