文献类型：期刊文献

英文题名：Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines

作者：Huang, Ding[1];Peng, Wen-Juan[1];Ye, Qian[1];Liu, Xu-Ping[1];Zhao, Liang[1];Fan, Li[1];Xia-Hou, Kang[1];Jia, Han-Jing[1];Luo, Jian[2];Zhou, Lin-Ting[2];Li, Bei-Bei[2];Wang, Shi-Lei[2];Xu, Wen-Ting[2];Chen, Ze[2];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Inst Biol Prod Co Ltd, Shanghai 200052, Peoples R China

年份：2015

卷号：10

期号：11

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000364303800023)】；

基金：This work was supported by the National Natural Science Foundation of China (Nos. 21206040, 21406066)(http://www.nsfc.gov.cn/), the National High Technology Research and Development Program of China (863 Program) (No. 2012AA02A303) (http://www.863.gov.cn/), the National Science and Technology Major Project (No. 2013ZX10004003-003-003) (http://www.nmp.gov.cn/), and the Fundamental Research Funds for the Central Universities (WF1214035) (http://jkw.mof.gov.cn/).

语种：英文

摘要：Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-containing and serum-free medium. It was found that the serum-free medium supported the stable subculture and growth of both adherent and suspension cells. In batch culture, for both cell lines, the growth kinetics in the serum-free medium was comparable with those in the serum-containing medium and a commercialized serum-free medium. In the serum-free medium, peak viable cell density (VCD), haemagglutinin (HA) and median tissue culture infective dose (TCID50) titers of the two cell lines reached 4.51x10(6) cells/mL, 2.94Log(10)(HAU/50 mu L) and 8.49Log(10)(virions/mL), and 5.97x10(6) cells/mL, 3.88Log(10)(HAU/50 mu L), and 10.34Log(10)(virions/mL), respectively. While virus yield of adherent cells in the serum-free medium was similar to that in the serum-containing medium, suspension culture in the serum-free medium showed a higher virus yield than adherent cells in the serum-containing medium and suspension cells in the commercialized serum-free medium. However, the percentage of infectious viruses was lower for suspension culture in the serum-free medium. These results demonstrate the great potential of this suspension MDCK cell line in serum-free medium for influenza vaccine production and further improvements are warranted.