文献类型：期刊文献

英文题名：pH-Responsive Fe3O4 Nanopartilces-Capped Mesoporous Silica Supports for Protein Delivery

作者：Gan, Qi[1,2];Zhu, Jiaoyang[2];Yuan, Yuan[1,2];Liu, Changsheng[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2016

卷号：16

期号：6

起止页码：5470

外文期刊名：JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY

收录：;EI(收录号：20162502514174);WOS:【SCI-EXPANDED(收录号:WOS:000386123900006)】；

基金：The authors wish to express their gratitude to the financial supports from National Basic Research Program of China (973 Program, No. 2012CB933600), the 111 Project (B14018), National Natural Science Foundation of China (Nos. 31100679, 31470924 and 31400817) and Chinese Postdoctoral Science Foundation (2013M541484). This study was also supported by Program for New Century Excellent Talents in University (No NCET-11-0640) and the national Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204).

语种：英文

外文关键词：pH-Responsive; Controlled Release; SBA-15; Fe3O4 Nanoparticles; Protein

摘要：Delivery of proteins and peptides with excellent bioactivity and controlled release still is a great challenge nowadays. In this study, a pH-responsive delivery system obtained by anchoring 8-nm Fe3O4 nanoparticles (NPs) onto SBA-15 supports with a particle diameter in the range of 0.6-1 mu m and a pore size of 6.2 nm was synthesized and investigated. The pH-stimulative response is based on the interaction between the tris(aminomethyl) ethane (TAE) groups anchored onto the pore outlet of mesoporous silica scaffolds and the carboxybenzaldehyde (CBA) groups coated on the Fe3O4 NPs, which can lead to a rapid release under the acid condition (pH=5) and a zero release with the increase of pH value (pH = 7.4). With BMP-2 as a model protein, this Fe3O4 nanopartilces-capped mesoporous silica showed a rapid response to the change of pH for protein delivery. Furthermore, the released BMP-2 could still maintain its bioactivity and induce the osteoblast differentiation of BMSCs. Besides, the magnetic orientation mainly attributes to the Fe3O4 NPs served as the nanocaps. The excellent bio-compatibility is demonstrated by the MTT assay on BMSCs model cells. These results show that Fe3O4 NPs-capped SBA-15 materials have an effective load for large molecule size proteins, such as BMP-2, and show an excellent applied prospect in pH-responsive controlled release system.