文献类型：期刊文献

英文题名：Avermectin induced DNA damage to the apoptosis and autophagy in human lung epithelial A549 cells

作者：Diao, Lin[1];Tang, Ning[1];Zhang, Cheng[2];Cheng, Jiagao[1];Zhang, Zhenhai[1];Wang, Siyu[1];Wu, Can[1];Zhang, Lutong[1];Tao, Liming[1];Li, Zhong[1];Zhang, Yang[1]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China;[2]UT Southwestern Med Ctr, Dept Pathol, Dallas, TX 75390 USA

年份：2021

卷号：215

外文期刊名：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000637943000006)】；

基金：This work was financial Sponsored by Shanghai Sailing Program (No. 19YF1411100) and Innovation Program of Shanghai Municipal Education Commission (No. 201701070002E00037) .

语种：英文

外文关键词：Avermectin; Cytotoxicity; DNA damage; Programmed cell death; Human A549 cells

摘要：Avermectin (AVM), as a biological insecticide, is widely used in agriculture and forestry production globally. However, inhalation of AVM may pose a risk, and the lung is the direct target, but the cytotoxicity of AVM on human lung cells is still unclear. Here, we attempted to elucidate the cytotoxic effect and molecular mechanism of AVM on human lung A549 cells. The results indicated that AVM inhibits cell proliferation, and enhances programmed cell death (apoptosis and autophagy). In addition, we found the AVM-treated cells showed an obvious drop in mitochondrial membrane potential and LC3-I/II, increased ROS production, DNA double-strand breaks, caspase-3/9 activated, PARP cleaved, cytochrome c and Bax/Bcl-2 content rise. The results showed that AVM induced mitochondria-related apoptosis and autophagy in lung A549 cells. These results indicate that AVM can pose a potential threat to human health by inducing DNA damage and programmed cell death.