文献类型：期刊文献

英文题名：Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris

作者：Xu, Yichun[1];Liu, Kunshan[1];Han, Yu[1];Xing, Yanzi[1];Zhang, Yuanxing[1];Yang, Qiuying[2];Zhou, Mian[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China

年份：2021

卷号：20

期号：1

外文期刊名：MICROBIAL CELL FACTORIES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000644646600001)】；

基金：This work was sponsored by the National Key Research and Development Program of China [2018YFA0900300], Shanghai Science and Technology Committee Rising-Star Program [19QA1402600] and Open Funding Project of the State Key Laboratory of Biocatalysis and Enzyme Engineering [SKLBEE2019010].

语种：英文

外文关键词：Codon bias; P; pastoris; Protein expression; Conformation

摘要：Background Protein synthesis is one of the extremely important anabolic pathways in the yeast expression system Pichia pastoris. Codon optimization is a commonly adopted strategy for improved protein expression, although unexpected failures did appear sometimes waiting for further exploration. Recently codon bias has been studied to regulate protein folding and activity in many other organisms. Results Here the codon bias profile of P. pastoris genome was examined first and a direct correlation between codon translation efficiency and usage frequency was identified. By manipulating the codon choices of both endogenous and heterologous signal peptides, secretion abilities of N-terminal signal peptides were shown to be tolerant towards codon changes. Then two gene candidates with different levels of structural disorder were studied, and full-length codon optimization was found to affect their expression profiles differentially. Finally, more evidences were provided to support possible protein conformation change brought by codon optimization in structurally disordered proteins. Conclusion Our results suggest that codon bias regulates gene expression by modulating several factors including transcription and translation efficiency, protein folding and activity. Because of sequences difference, the extent of affection may be gene specific. For some genes, special codon optimization strategy should be adopted to ensure appropriate expression and conformation.