文献类型：期刊文献

英文题名：Genome-based analysis to understanding rapid resuscitation of cryopreserved anammox consortia via sequential supernatant addition

作者：Zhou, Yuan-yuan[1,2];Shao, Wen-li[1,2];Liu, Yong-di[1,2,3];Li, Xiang[4];Shan, Xiao-yu[5];Jin, Xin-bai[1,2];Gao, Jie[1,2];Li, Wei[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Sch Resources & Environm Engn, Natl Engn Lab Ind Wastewater Treatment, Shanghai, Peoples R China;[3]Shanghai Inst Pollut Control & Ecol Secur, Shanghai, Peoples R China;[4]Suzhou Univ Sci & Technol, Suzhou, Peoples R China;[5]MIT, Dept Civil & Environm Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA

年份：2020

卷号：744

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20203008962025);WOS:【SCI-EXPANDED(收录号:WOS:000573549300007)】；

基金：This work was supported by National Natural Science Foundation of China (51808223), Fundamental Research Funds for the Central Universities (JKB012014053, 50321022017009), National Key Research and Development Program of China (2019YFC0408202), Shanghai Educational Development Foundation (18CG34), Shanghai Rising -Star Program (20QC1400500). The authors would like to thank Muhammad Asif for language polish at an earlier version of the manuscript.

语种：英文

外文关键词：Cryopreserved anammox consortia; Bio-augmentation; Metatranscriptomics; Cross-feeding

摘要：Simple cryopreservation of anaerobic ammonium-oxidation (anammox) consortia has become a promising preservation technology for the fast start-up of the anammox process. Here, we use genome-resolved metagenomics and metatranscriptomics to understand of the microbial interaction in a simple and effective resuscitation process for long-term cryopreserved anammox consortia by sequential addition of anammox SBR supernatant. Performance results showed that sequential addition of anammox supernatant significantly reduced the resuscitation time of the granule-based anammox process from 40 to 20 days. Genome-centric metagenomics were used to recover 19 high-quality draft genomes of anammox and heterotrophic bacteria. Comparative metatranscriptomic analysis was conducted to examine the gene expression of Candidatus Kuenenia stuttgartiensis, the dominant anammox bacterium, and heterotrophic bacteria to better understand their potential interactions. Proteobacteria-affiliated bacteria found in the supernatant were highly active in producing the secondary metabolites molybdopterin cofactor and folate which are needed for growth of the auxotrophic anammox bacteria. In addition, the significantly higher expression levels of hzsA and CO2-fixtion genes in the Candidatus Kuenenia genome indicated the anammox bacteria were likely more active and growing faster after sequential anammox supernatant addition during the resuscitation process. The resuscitation treatment pulse assays confirmed that sequential addition of supernatant was an effective way for the rapid resuscitation of anammox consortia. Our findings offer the first evidence of cross-feeding during the rapid resuscitation of cryo-preserved anammox consortia. (C) 2020 Elsevier B.V. All rights reserved.