文献类型：期刊文献

英文题名：Transcriptional and post-translational regulation of AccD6 in Mycobacterium smegmatis

作者：Liu, Xin-Xin[1];Shen, Meng-Jia[1];Liu, Wei-Bing[1];Ye, Bang-Ce[1,2]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Meilong Rd 130, Shanghai 200237, Peoples R China;[2]Shihezi Univ, Sch Chem & Chem Engn, Xinjiang, Peoples R China

年份：2018

卷号：365

期号：9

外文期刊名：FEMS MICROBIOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000441111700010)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (31730004, 21335003 and 21575089).

语种：英文

外文关键词：acetyl-CoA (propionyl-CoA) carboxylase; transcriptional regulation; acetylation (propionylation) regulation

摘要：AccD6 is an important component of acetyl-CoA/propionyl-CoA carboxylase, which acts as a key role in mycolic acid synthesis and short chain fatty acyl-coenzyme A metabolism. In this study, we demonstrated that AccD6 of Mycobacterium smegmatis associates with AccA3 (alpha subunit of acetyl-CoA carboxylase, MSMEG_1807) and AccE (epsilon subunit, MSMEG_1812) to form the acetyl-CoA (propionyl-CoA) carboxylase. Results showed that the MSMEG_4331 subunit is a regulator that interacts with the promoter region of accD6 to inhibit its transcription. Transcription of accD6 was reduced by 50% in the mutant M. smegmatis strain overexpressing MSMEG_4331. Moreover, the activity of AccD6 was inhibited by acylation (such as acetylation and propionylation). These results demonstrate that AccD6 of M. smegmatis is regulated at both the transcriptional and post-translational levels. Our findings highlight the novel regulatory mechanism underlying mycolic acid biosynthesis in mycobacteria.