文献类型：期刊文献

英文题名：Spatiotemporal Controlled Formation of Synthetic Nanoreactors in Single Living Cells

作者：Lv, Jian[1];Wang, Xiao-Yuan[1];Zhou, Xin-Yue[1];Wu, Xue[1];Chang, Shuai[1];Chen, Bin-Bin[2];Guo, Zhi-Qian[1];Li, Da-Wei[1];Qian, Ruo-Can[1]

机构：[1]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Key Lab Adv Mat,Joint Int Lab, Shanghai 200237, Peoples R China;[2]Chinese Univ Hong Kong, Shenzhen Inst Aggregate Sci & Technol, Sch Sci & Engn, Shenzhen 518172, Guangdong, Peoples R China

年份：2023

卷号：15

期号：44

起止页码：50854

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20234815127466);WOS:【SCI-EXPANDED(收录号:WOS:001092779100001)】；

基金：This research was supported by the National Natural Science Foundation of China (21977031, 22176058, 21974046), the China Postdoctoral Science Foundation (2021M701195), Shanghai Science and Technology Committee (22ZR1416800 and 23ZR1416100), the Program of Introducing Talents of Discipline to Universities (B16017), and the Fundamental Research Funds for the Central Universities (222201717003). The authors thank Research Center of Analysis and Test of East China University of Science and Technology for the help on the characterization.

语种：英文

外文关键词：synthetic nanoreactors; single cell; ZIF-8nanoparticles; photocatalytic degradation; nanopipette

摘要：Cellular compartments provide confined environments for spatiotemporal control of biological processes and enzymatic reactions. To mimic such compartmentalization of eukaryotic cells, we report an efficient and general platform to precisely control the formation of artificial nanoreactors in single living cells. We introduce an electroosmotic controlled strategy for the synthesis of ZIF-8 at the nanoscale liquid-liquid interface around the tip of a nanopipet, whereby the formed ZIF-8 nanoparticles are driven into a single living cell by the electroosmotic flow. The porous ZIF-8 nanoparticles, as synthetic nanoreactors, are not only able to harvest fluorescent molecules from peripheral cytoplasm but also perform the subsequent photocatalytic degradation, mimicking compartmentalized chemical reactions in eukaryotic cells. Our strategy provides a useful tool for spatiotemporal controlled synthesis of artificial nanoreactors with on-demand functions in single living cells with versatile applications in chemical biology.