文献类型：期刊文献

中文题名：乙二胺羟丙基壳聚糖固定化天门冬酰胺酶的反应性质

英文题名：Reaction Properties for the Immobilized L-asparaginase with Ethylenediamine-Hydroxypropyl Chitosan

作者：方波[1];霍红光[1];宋道云[1];江体乾[1]

机构：[1]华东理工大学化学工程研究所,上海200237

年份：2002

卷号：28

期号：5

起止页码：482

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;EI(收录号：2002507271797);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目 (2 980 60 0 6)

语种：中文

中文关键词：L-天门冬酰胺酶;固定化酶;反应动力学;壳聚糖胺基衍生物;酶活力;乙二胺羟丙基壳聚糖

外文关键词：L;asparaginase; immobilized enzyme; reaction kinetics; aminated chitosan derivative

摘要：研究了乙二胺羟丙基壳聚糖固定化 L -天门冬酰胺酶反应动力学性能。分别考察了固定化酶耐胃蛋白酶性能、保存条件对固定化酶活力的影响、固定化酶的米氏常数 Km以及固定化酶在缓冲液中间歇催化底物和连续催化底物的反应动力学性能。结果表明 ,在适当的间歇和连续反应条件下 。
The reaction properties for the immobilized L aspara ginase with ethylenediamine hydroxypropyl chitosan were studied in this paper. The effects of store condition on the activity of the immobilized enzyme was inv estigated.For the immobilized enzyme,the K m value, the property of resisting protease, the bath reaction kinetics and the continuous reaction kinetics in bu ffers were studied. The results show that, under the proper react ion condition, the immobilized enzyme posseses good properties for hydrating asparagin both in batch and in continuous type.