文献类型：期刊文献

英文题名：Enhancing cordycepin production in liquid static cultivation of Cordyceps militaris by adding vegetable oils as the secondary carbon source

作者：Tang, Jiapeng[1];Qian, Zhenqing[1];Wu, Hui[2,3,4]

机构：[1]Nantong Univ, Inst Naut Med, Dept Biochem & Pharm, Nantong 226001, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]China Natl Light Ind Council, Key Lab Biobased Mat Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：268

起止页码：60

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20183105637292);WOS:【SCI-EXPANDED(收录号:WOS:000445043600008)】；

基金：This work was supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (16KJB530004), Applied Research Program of Nantong City (MS12015066), Natural Science Research Program of Nantong University (13ZJ006), Natural Science Pre-research Project of Nantong University and Scientific Research Foundation for introduced talents of Nantong University.

语种：英文

外文关键词：Cordyceps militaris; Cordycepin production; Peanut oil; Liquid static cultivation; Carbon metabolism

摘要：This study evaluated different vegetable oils as the second carbon source in liquid static culture of Cordyceps militaris in terms of mycelial growth and cordycepin production. The maximum mycelial concentration and cordycepin production were observed under cottonseed oil and peanut oil induction, respectively. In the condition of adding 20 g/L of peanut oil at Day 0, the final concentration of cordycepin reached to the highest, about 5.29 g/L, which was about 3.17 times higher than that of the control. The qRT-PCR and enzyme activity analysis confirmed that addition of peanut oil up-regulated the expression of the genes encoding glucose-6-phosphate dehydrogenase and isocitrate lyase, as well as the genes in the cordycepin biosynthesis pathway, cns1 and cns2, during the cultivation in C. militaris.