文献类型：期刊文献

英文题名：Metabolic Dynamic Regulation Analysis of Ammonium Induced Efficient Nemadectin Biosynthesis of Streptomyces Cyaneogriseus

作者：Zhang, Zishu[1]; Song, Xiaoqing[1]; Zhang, Yun[1]; Xue, Jiayun[1]; Yu, Junxiong[1]; Wang, Zejian[1]; Moshin, Ali[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, China State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai 200240, China

年份：2025

外文期刊名：SSRN

收录：EI(收录号：20250083867)

语种：英文

外文关键词：Biosynthesis - Gene expression - Gene expression regulation - Metabolism - Metabolites

摘要：Nemadectin is a commercialized antibiotic which effectively eradicates pests and suitable for large-scale production. Ammonium regulation enhanced nemadectin biosynthesis by 120% in Streptomyces cyaneogriseus. Integrated transcriptomic and metabolomic analyses revealed upregulated nemadectin biosynthetic genes, enhanced precursor pathways and altered metabolic fluxes (increased glycolysis, decreased TCA cycle). These insights into gene expression, metabolite dynamics and regulatory factors provide a foundation for optimizing fermentation and genetic engineering in S. cyaneogriseus. Multi-omics analysis contribute to the understanding of nemadectin synthesis mechanism. ? 2025, The Authors. All rights reserved.