文献类型：期刊文献

英文题名：Androglobin knockdown inhibits growth of glioma cell lines

作者：Huang, Bo[2];Lu, Yi-Sheng[2];Li, Xia[3];Zhu, Zhi-Chuan[1];Li, Kui[1];Liu, Ji-Wei[1];Zheng, Jing[1];Hu, Ze-Lan[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[2]PLA, Dept Orthopaed 2, Hosp 117, Hangzhou, Zhejiang, Peoples R China;[3]Yi Ji Shan Hosp, Wan Nan Med Coll, Wuhu, Anhui, Peoples R China

年份：2014

卷号：7

期号：5

起止页码：2179

外文期刊名：INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000338770300033)】；

基金：This work was supported by the Exploratory Research Foundation of ECUST to Zelan Hu and Jing Zheng, and by the Shanghai Committee of Science and Techonology (Grant 11DZ226 06-00).

语种：英文

外文关键词：Androglobin; glioma; proliferation; apoptosis

摘要：Globin family was famous for oxygen supply function of its members such as hemoglobin and myoglobin. With the progress of research, several members of this protein family have been proven to play roles in tumors including glioma. Androglobin (ADGB) is a recently identified member of globin family with very few studies about its function. In the present study, we show that ADGB plays an oncogene role in glioma. Lentiviral vector mediated ADGB knockdown inhibited the proliferation of glioma cell lines determined by MTT assay and colony formation assay. ADGB knockdown also increased the apoptosis of glioma cell line U251 assessed by flow cytometry. In addition, western blot showed that ADGB knockdown altered levels of several proteins related to proliferation, survival or apoptosis in U251 cells. These findings suggest ADGB is involved in the progression of glioma in vitro.