文献类型：期刊文献

英文题名：One-Step Click Engineering Considerably Ameliorates the Practicality of an Unqualified Rhodamine Probe

作者：Li, Kai-Bin[1,2];Wang, Huan[1,2,3];Zang, Yi[3];He, Xiao-Peng[1,2];Li, Jia[3];Chen, Guo-Rong[1,2];Tian, He[1,2]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China

年份：2014

卷号：6

期号：22

起止页码：19600

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20145000309387);WOS:【SCI-EXPANDED(收录号:WOS:000345721400021)】；

基金：We thank the 973 project (2013CB733700), the National Science Fund for Distinguished Young Scholars (81125023), the National Natural Science Foundation of China (21176076, 21202045, and 91213303), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA01040303), Program of Shanghai Subject Chief Scientist (13XD1404300), the Key Project of Shanghai Science and Technology Commission (13NM1400900), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：click chemistry; rhodamine; probe; triazole; PEG; cell imaging

摘要：This study describes the exploitation of click chemistry in the one-step molecular engineering of an unqualified rhodamine probe, leading to its considerable functional enhancement in terms of water solubility, ion selectivity, and usefulness in detecting biological and environmental samples. A dipropargyl rhodamine dye previously identified as an unselective and poorly water-soluble mercury(II) probe was used to couple with an azido polyethylene glycol (PEG) by the Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition click reaction in almost quantitative yield. The simple click-engineered rhodamine probe shows, remarkably, better water solubility and mercury(II) selectivity comparing to the raw counterpart, and can be used to sensitively image mercury ions internalized by live cells and to accurately quantify the ion spiked in river water specimens. This study provides insights into the simple functional improvement of unqualified molecular dye probes via the efficient "click engineering".