文献类型：期刊文献

英文题名：Protection of a CpG-adjuvanted DNA vaccine against infectious hematopoietic necrosis virus (IHNV) nature infection in rainbow trout (Oncorhynchus mykiss)

作者：Wu, Di[1];Shao, Shuai[1];Liu, Qin[1,3];Wang, Qiyao[1,3];Zhang, Yuanxing[2,3];Liu, Xiaohong[1,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2023

卷号：572

外文期刊名：AQUACULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000981145700001)】；

基金：This work was supported by the grants of National Natural Science Foundation of China (32025038), China Agriculture Research System of MOF and MARA (CARS-47) and the Fundamental Research Funds for the Central Universities (222201211716).

语种：英文

外文关键词：CpG-ODN; DNA vaccine; Field trial; Infectious hematopoietic necrosis virus; Oncorhynchus mykiss; Rainbow trout

摘要：Salmonid larvae, especially rainbow trout larvae, are suffering from disease caused by infectious hematopoietic necrosis virus (IHNV) with high mortality worldwide. As well, IHNV strains had been isolated from rainbow trout in China and caused huge loss. Since there had been no commercial vaccine to control IHNV outbreaks in China, a DNA vaccine encoded glycoprotein based on a local isolated IHNV strain was developed with a series of CpG sequence used as adjuvant, and the protection of the vaccine under natural culture condition was carried out. As a result, a high survival rate of 85% was obtained in vaccinated fish compared with 60% in untreated group after a serious nature infection. An innate immune response was induced in the early state of infection represented by significant up-regulations of Mx-1 and IFN-gamma, resulting in a low IHNV load in vaccinated fish. Moreover, specific antibody of IgM and neutralizing antibody (NAb) was provoked after the vaccination and expressions of CD4 and CD8 were gradually increased. These results suggested that both innate and adaptive immunity were induced by the DNA vaccine during the field trial, which may guide for further control of IHNV outbreaks in farm rainbow trout culture.