文献类型：期刊文献

英文题名：Key role of exopolysaccharide on di-butyl phthalate adsorbing by Lactobacillus plantarum CGMCC18980

作者：Fan, Yu-Hang[1];Shen, Yi-Lin[1];Lin, Zhi-Wei[1];Zhou, Ying[1];Ye, Bang-Ce[2]

机构：[1]East China Univ Sci & Technol, Sch Bioengn, Dept Food Sci & Technol, Meilong RD 130, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Meilong RD 130, Shanghai 200237, Peoples R China

年份：2021

卷号：105

期号：6

起止页码：2587

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20211110069077);WOS:【SCI-EXPANDED(收录号:WOS:000625624900001)】；

基金：This research was supported and funded by Science and Technology Commission of Shanghai Municipality (grant number: 18JC1410802).

语种：英文

外文关键词：Lactobacillus plantarum; Di-butyl phthalate; Adsorption; Estrogen

摘要：Plasticizers belong to hormone-like substances existing widely in the environment. According to the Environmental Protection Agency of China, they are considered to be the fourth class of toxic chemicals due to their harmful effects on normal endocrine system in human bodies. In the recent published work of our lab, Lactobacillus plantarum CGMCC18980 (strain P1) could reduce the toxicity of di-butyl phthalate (DBP) in rats effectively. The purpose of this study is to further explore the adsorption mechanism of di-butyl phthalate to L. plantarum CGMCC18980, based on optimizing the adsorption conditions. As a consequence, the adsorption effect of L. plantarum CGMCC18980 attributed to relationships between exopolysaccharide, membrane protein, and the cell wall. Experimental results demonstrated that exopolysaccharide and the cell wall were devoted to DBP binding. An obvious adsorption layer was observed outside of L. plantarum CGMCC18980 through scanning electron microscope (SEM) and transmission electron microscope (TEM). The Fourier transform infrared spectroscopy (FTIR) results showed that the functional groups involved in adsorption were mainly C=O, C-N, and C-O, which related to lipids and polysaccharides. Zeta potential analysis indicated that DBP adsorption had no significant relationship with surface charge. These results revealed that exopolysaccharide may be the key factor of strain CGMCC18980 in DBP adsorption.