文献类型：期刊文献

英文题名：Triple-gene deletion mutant (zmlaEzompRzdeoR) attenuates Pseudomonas plecoglossicida and elicits effective protective immunity in large yellow croaker

作者：Ahat, Peherden[1];Zhang, Zengjie[1];Song, Wei[2];Liu, Qin[1,3,4];Zhang, Yibei[1,3,4];Wang, Qiyao[1,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Fishery Sci, Minist Agr, East China Sea Fisheries Res Inst, Key Lab Ocean & Polar Fisheries, Shanghai 200090, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]Lab Aquat Anim Dis MARA, Shanghai 200237, Peoples R China

年份：2026

卷号：176

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001806357900001)】；

基金：This research was funded by the National Key Research and Development Program of China (2023YFD2400702) and China Agriculture Research System of MOF and MARA (CARS-46).

语种：英文

外文关键词：Pseudomonas plecoglossicida; Large yellow croaker ( Larimichthys crocea ); Vaccine; Virulence

摘要：Visceral white nodules disease (VWND), caused by Pseudomonas plecoglossicida, poses a significant threat to the large yellow croaker (Larimichthys crocea) aquaculture industry. Developing live attenuated vaccines (LAVs) requires a fine balance between safety and immunogenicity, which remains a primary challenge. In this study, we developed a stable and effective LAV by constructing a markerless triple-gene deletion mutant zmlaEzompRzdeoR of P. plecoglossicida. The mutant exhibited high genetic stability and maintained growth kinetics compared to the wild-type (WT) strain under host-relevant conditions. Vaccination trials demonstrated that the triple mutant was significantly attenuated and safe for L. crocea. Following WT challenge, the triple mutant provided a relative percent survival (RPS) of 80.43% via intraperitoneal (i.p.) injection and 76.92% via immersion. For i. p.-vaccinated group, a significant reduction in splenic bacterial colonization and the absence of macroscopic white nodules were observed post-challenge. Furthermore, i. p.-vaccination triggered a coordinated immune response, characterized by the early upregulation of pro-inflammatory cytokines followed by the sustained activation of adaptive immune-related genes and elevated serum-specific IgM levels. These findings demonstrate that the zmlaEzompRzdeoR mutant is a promising LAV candidate, providing a viable strategy for the integrated control of P. plecoglossicida infection.