文献类型：期刊文献

中文题名：甲醛活化壳聚糖制备固定化l-天冬酰胺酶

英文题名：Immobilization of l Asparaginase on Chitosan Activated by Formaldehyde

作者：周纪宁[1];江体乾[1];方波[1];李永丰[1]

机构：[1]华东理工大学化工所流变学研究室

年份：1998

卷号：11

期号：4

起止页码：534

中文期刊名：功能高分子学报

外文期刊名：Journal of Functional Polymers

收录：CSTPCD;;CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：壳聚糖;天冬酰胺酶;固定化酶;抗白血病药

外文关键词：Chitosan,l Asparaginase,Immobilized enzyme, Immobilization

摘要：研究了以甲醛活化的壳聚糖为载体固定化ｌ天冬酰胺酶，其活力回收可达到１１３％，比用以戊二醛为交联剂进行固定化的活力回收要高出五倍多。分别从缓冲液的ｐＨ值及甲醛用量等方面进行了固定化ｌ－天冬酰胺酶的条件优化，并且对固定化酶的性质作了探讨。
 asparaginase was immobilized on chitosan activated by formaldehyde. Experiments showed that the activity retention could reach 113%, which was nearly five times the activity of immobilized l asparaginase by using glutaraldehyde as crosslinking reagent.The optimum immobilization conditions were obtained by investigating the buffer pH value and formaldehyde dosage. The properties of immobilized l asparaginase were also studied.