文献类型：期刊文献

英文题名：Purification and Identification of Flavonoids from Prunus mume and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes

作者：Liu, Yuxuan[1];He, Mengdie[2];Wei, Xinxin[1];Han, Qifeng[1];Yang, Xiang[1];Huang, Gangyi[2];Zhang, Chen[2];Wu, Mengqi[1];Zhang, Wenqing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Zhejiang YingShu Biol Technol Co Ltd, Hangzhou 311215, Peoples R China

年份：2025

卷号：12

期号：1

外文期刊名：COSMETICS

收录：WOS:【ESCI(收录号:WOS:001429648800001)】；

基金：This research was funded by the Science and Technology Commission of Shanghai Municipality, grant number 22S21902800.

语种：英文

外文关键词：Prunus mume; extraction; purification; UHPLC-DAD-QTOF-MS/MS; anti-photoaging activity; human keratinocytes (HaCaT)

摘要：Prunus mume (Armeniaca mume Sieb. var. mume f. viridicalyx (Makino) T. Y. Chen) is a common landscape plant. It has rich flavonoid content, which shows strong biological activities. In this study, the application potential of flavonoids in Prunus mume in anti-photoaging cosmetics was developed. Firstly, the flavonoids in Prunus mume were extracted by the heating reflux method, and the total flavonoid content reached 192 mg/g under the optimal process conditions determined by orthogonal testing. Then, the crude extract (PMCE) was purified by NKA-II resin column chromatography, and the purity of the flavonoids reached 83.99%. Subsequently, the Prunus mume purified product (PMPP) was analyzed by UHPLC-DAD-QTOF-MS/MS and 45 compounds were identified including flavonoid glycosides, phenolic acids and p-coumaroyl acetylated sucrose. In addition, in vitro biological experiments showed that PMPP had significant repair effects on UVB-induced photoaging of human keratinocytes (HaCaT), lowering reactive oxygen species (ROS) and malondialdehyde (MDA) levels, restoring superoxide dismutase (SOD) activity, and promoting hyaluronic acid (HA) secretion and Aquaporins-3 (AQP-3) levels. The results laid the foundation for the development of the flavonoids in Prunus mume as materials for anti-photoaging cosmetics.