文献类型：期刊文献

英文题名：Metabolic Engineering for the Comprehensive Utilization of N,N-Dimethylformamide-Containing Wastewater

作者：Chen, Yumeng[1];Wang, Meng[1];Wang, Wei[1,3];Zeng, Yi[1];Fan, Xingjia[1];Tu, Maobing[2];Ma, Yushu[1];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Univ Cincinnati, Dept Chem & Environm Engn, Cincinnati, OH 45221 USA;[3]Jiangsu YIMING Biol Technol CO LTD, Suqian City 223699, Jiangsu, Peoples R China

年份：2022

卷号：70

期号：42

起止页码：13574

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20224212971685);WOS:【SCI-EXPANDED(收录号:WOS:000870248500001)】；

基金：Science Foundation of Shanghai (grant number 22ZR1417600) . Notes The authors declare no competing financial interest. Funding This research was supported by the Shanghai Agriculture Applied Technology Development Program, China (grant number 2021-02-08-00-12-F00758) , National Natural Science Foundation of China (grant number 32000050) , and Natural Science Foundation of Shanghai (grant number 22ZR1417600) .

语种：英文

外文关键词：cellulase; N; N-dimethylformamide; separate enzymatic hydrolysis and fermentation; succinate; succinic acid; cellulosic sugar

摘要：N, N-dimethylformamide is frequently present in industrial wastewater and is environmentally detrimental. The current study aims to assess the utilization and biodegradation of N, N-dimethylformamide-containing wastewater to lessen the associated environmental load. Results show that addition of wastewater containing N, N-dimethylformamide to Trichoderma reesei fermentation media enhances cellulase production and facilitates cellulose hydrolysis. However, N, N-dimethylformamide is a cellulase enhancer that is not degraded during cellulase production in T. reesei fermentation and is retained in the N, N- dimethylformamide-enhanced cellulase solution. Indeed, the cellulosic sugar solution generated via lignocellulose hydrolysis with N, N-dimethylformamide-enhanced cellulase retains N, N-dimethylformamide. We further identified three core enzyme modules -N, N-dimethylformamidase, dimethylamine dehydrogenase, and methylamine dehydrogenase enzyme -which were inserted into Escherichia coli to develop metabolically engineered strains. These strains degraded N, N-dimethylformamide and produced succinate using N, N-dimethylformamide-enhanced cellulosic sugar as the substrate. The platform described here can be applied to effectively convert waste into valuable bioproducts.