文献类型：期刊文献

英文题名：Anaerobic hydrocarbon degradation in candidate phylum 'Atribacteria' (JS1) inferred from genomics

作者：Liu, Yi-Fan[1,2];Qi, Zhen-Zhen[1,2];Shou, Li-Bin[1,2];Liu, Jin-Feng[1,2];Yang, Shi-Zhong[1,2];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[3]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2019

卷号：13

期号：9

起止页码：2377

外文期刊名：ISME JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000482118300019)】；

基金：The authors thank Jiaqi Zhao, Chenyu Ge, Yi Zhang, Ting Han and Di Wang for annotation the genome bins, we also thank Donovan H. Parks for kindly sharing the unpublished genome bins. This work was supported by grants of National Natural Science Foundation of China (Grant No. 41530318, 41373070, 41807324), the Fundamental Research Funds for the Central Universities (No. 22221818014, 50321101917017) and the Research Program of State Key Laboratory of Bioreactor Engineering to YFL, ZZQ, LBS, SZY, JFL and BZM and the NSFC/RGC Joint Research Fund (No. 41161160560) to JDG.

语种：英文

摘要：The hydrocarbon-enriched environments, such as oil reservoirs and oil sands tailings ponds, contain a broad diversity of uncultured microorganisms. Despite being one of the few prokaryotic lineages that is consistently detected in both production water from oil reservoirs and stable hydrocarbon-degrading enrichment cultures originated from oil reservoirs, the physiological and ecological roles of candidate phylum "Atribacteria" (OP9/JS1) are not known in deep subsurface environments. Here, we report the expanded metabolic capabilities of Atribacteria as inferred from genomic reconstructions. Seventeen newly assembled medium-to-high-quality metagenomic assembly genomes (MAGs) were obtained either from co-assembly of two metagenomes from an Alaska North Slope oil reservoir or from previous studies of metagenomes coming from different environments. These MAGs comprise three currently known genus-level lineages and four novel genus-level groups of OP9 and JS1, which expands the genomic coverage of the major lineages within the candidate phylum Atribacteria. Genes involved in anaerobic hydrocarbon degradation were found in seven MAGs associated with hydrocarbon-enriched environments, and suggest that some Atribacteria could ferment short-chain n-alkanes into fatty acid while conserving energy. This study expands predicted metabolic capabilities of Atribacteria (JS1) and suggests that they are mediating a key role in subsurface carbon cycling.