文献类型：期刊文献

英文题名：Donor-conformation-dependent energy transfer for dual-color fluorescent probe with high-resolution imaging

作者：Guo, Lifang;Jin, Xin;Liu, Hao;Zhu, Jiangnan;Zhang, Zhiyun[1];Su, Jianhua

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Frontiers Sci Ctr Materiobiol & Dynam Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Shanghai 200237, Peoples R China; East China Univ Sci & Technol, Joint Int Res Lab Precis Chem & Mol Engn, Frontiers Sci Ctr Materiobiol & Dynam Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Shanghai 200237, Peoples R China

年份：2021

卷号：64

期号：8

起止页码：1310

外文期刊名：SCIENCE CHINA-CHEMISTRY

收录：;EI(收录号：20211710243922);WOS:【SCI-EXPANDED(收录号:WOS:000640945900001)】；

基金：This work was supported by the National Natural Science Foundation of China (21788102, 21905090, 21790361, 22004036, 91859205), Shanghai Municipal Science and Technology Major Project (2018SHZDZX03), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, Programme of Introducing Talents of Discipline to Universities (B16017), Shanghai Science and Technology Committee (17520750100), Natural Science Foundation of Shanghai (19ZR1412200), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：VIE; FRET; high-resolution; dual-color; large Stokes shift

摘要：Herein, we presented a brand-new concept to construct the Forster resonance energy transfer (FRET) based cassette by integrating a vibration-induced emission (VIE) chromophore as the donor. Different from traditional donors only with a single emission, the VIE donor possessed well-separated dual emission bands by altering the excited state molecular configuration from bent state to the planar state. By linking an acceptor such as a cyanine dye (Cy5), a novel VIE-FRET cassette (PPCy5) was prepared. The planar emission profile of VIE donor moiety could fully cover the absorption of Cy5, thus the complete FRET process enabled the excellent bimodal spectra difference of 142 nm and ultra-large pseudo-Stokes shift of up to 300 nm. Benefiting from the viscosity-dependent characteristic of the VIE donor, PPCy5 could clearly and intuitively reveal the different viscosity regions in vivo by dual-color and high-resolution imaging. The VIE-FRET paradigm provides an optional platform for developing donor-acceptor based dual-color fluorescent probes with high-resolution imaging ability.