文献类型：期刊文献

英文题名：Single-visible-light performed STORM imaging with activatable photoswitches

作者：Li, Fanghui[1,2];Li, Mengqi[1,2];Shi, Yiqi[1,2];Bian, Xinyun[1,2];Lv, Ning[1,2];Guo, Shaomeng[1,2];Wang, Ying[1,2];Zhao, Weijun[1,2,3];Zhu, Wei-Hong[1,2,3]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Joint Int Res Lab Precis Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr, Shanghai Key Lab Funct Mat Chem,Inst Fine Chem, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Ctr Photosensit Chem Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：16

期号：31

起止页码：14270

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20252818758281);WOS:【SCI-EXPANDED(收录号:WOS:001525106500001)】；

基金：This work was supported by the National Key Research and Development Program of China (2021YFA0910000); Basic Science Center of National Natural Science Foundation (T2488302); National Natural Science Foundation of China (9235630033, 22338006, 22105069); Science and Technology Commission of Shanghai Municipality (grant no. 24DX1400200); and Innovation Program of Shanghai Municipal Education Commission (2023FGS01). The authors thank the staff members of the Integrated Laser Microscopy System at the National Facility for Protein Science in Shanghai (NFPS), Shanghai Advanced Research Institute, Chinese Academy of Sciences, China for data collection and analysis. The authors are grateful to Lili Wang from Nikon Precision (Shanghai) Co., Ltd for useful assistance on the NIKON N-STORM.

语种：英文

外文关键词：Biomolecules - Diffraction - Fluorescence - Optical resolving power - Photochromism - Storms

摘要：Stochastic optical reconstruction microscopy (STORM) overcomes the diffraction limit of optical imaging, facilitating high-resolution visualization of cellular substructures at the nanoscale. Essential to this technique is the development of fluorescent photoswitches. However, existing photoswitches typically rely on sophisticated dual-beam systems that involve harmful UV-light and lack specific recognition of biomolecules. Here we develop unique intracellular biomolecule-activatable photoswitches tailored for single-visible-light performed STORM imaging. Upon incorporating intramolecular proton transfer (IPT) units into the photochromic diarylethene, the all-visible-light driven photoswitches are established with excellent photoresponsive efficiency, high brightness and fluorescence ON-to-OFF contrast ratio, guaranteeing STORM imaging using a single-visible-light (488 nm) by regulating the activation, excitation and deactivation processes. Furthermore, we functionalized the IPT units with biomolecular recognition motifs, creating photoswitches capable of sensing the expression levels of intracellular biomolecules (like glutathione (GSH) or beta-galactosidase (beta-Gal)) with super-resolution. Our objective is to engineer single-visible-light driven, biomolecule-activatable photoswitches, which will significantly streamline the STORM technique and expand the applicability of super-resolution imaging for the precise mapping of intracellular substructures.