文献类型：期刊文献

英文题名：Very High Thermotolerance of an Adaptive Evolved Saccharomyces cerevisiae in Cellulosic Ethanol Fermentation

作者：Zhang, Bin[1];Geberekidan, Mesfin[1];Yan, Zhao[1];Yi, Xia[2];Bao, Jie[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Changzhou Univ, Natl Local Joint Engn Res Ctr Biomass Refining & H, Changzhou 213164, Peoples R China

年份：2023

卷号：9

期号：4

外文期刊名：FERMENTATION-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000977149600001)】；

基金：This research was supported by the National Natural Science Foundation of China (21978083, 31961133006), and the Yangfan Project of Science and Technology Committee of Shanghai Municipality (23YF1409900).

语种：英文

外文关键词：thermotolerance; adaptive evolution; Saccharomyces cerevisiae; cellulosic ethanol

摘要：High thermotolerance is an important property of Saccharomyces cerevisiae for stable and efficient bioethanol production, especially for large-scale bioethanol production with weak heat transfer and the simultaneous saccharification and fermentation (SSF) of lignocellulosic biomass at high temperatures (above 40 degrees C). Despite extensive studies involving metabolic engineering and chemical mutagenesis, the improvement of thermotolerance in S. cerevisiae under harsh thermal stress (42-45 degrees C) has been limited. A highly thermotolerant strain, S. cerevisiae Z100, by a 91 days' laboratory adaptive evolution in wheat straw hydrolysate was applied for cellulosic ethanol fermentation. The results showed that the cell survival ratio of S. cerevisiae Z100 at 50 degrees C improved by 1.2 times that of the parental strain. The improved thermotolerance of S. cerevisiae Z100 at 50 degrees C was found to contribute significantly to enhanced cellulosic ethanol fermentability. The ethanol production of S. cerevisiae Z100 increased by 35%, 127%, and 64% when using wheat straw as feedstock after being maintained at 50 degrees C for 12 h, 24 h, and 48 h, respectively. Transcriptome analyses suggested that the enhanced trehalose and glycogen synthesis, as well as carbon metabolism, potentially contributed to the improved thermotolerance and the ethanol fermentability of S. cerevisiae Z100. This study provides evidence that adaptive evolution is an effective method for increasing the thermotolerance of the S. cerevisiae strain for stable and efficient cellulosic ethanol production.