文献类型：期刊文献

英文题名：Blocking the flow of propionate into TCA cycle through a mutB knockout leads to a significant increase of erythromycin production by an industrial strain of Saccharopolyspora erythraea

作者：Chen, Chongchong[1];Hong, Ming[1];Chu, Ju[1];Huang, Mingzhi[1];Ouyang, Liming[1];Tian, Xiwei[1];Zhuang, Yingping[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,POB 329, Shanghai 200237, Peoples R China

年份：2017

卷号：40

期号：2

起止页码：201

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;EI(收录号：20164202909075);WOS:【SCI-EXPANDED(收录号:WOS:000394371000004)】；

基金：This work was financially supported by a Grant from the Major State Basic Research Development Program of China (973 Program, No. 2012CB721006) and National Natural Science Foundation of China (No. 21276081).

语种：英文

外文关键词：Saccharopolyspora erythraea; Erythromycin; N-propanol; Methylmalonyl-CoA mutase; mutB

摘要：A high erythromycin producing mutant strain Saccharopolyspora erythraea HL3168 E3-Delta mutB was constructed by deleting mutB (SACE_5639) gene encoding the beta subunit of methylmalonyl-CoA mutase of an industrial strain of S. erythraea HL3168 E3. Industrial media and process control strategies were adopted in a 5 L bioreactor for characterizing the physiological parameters. The total erythromycin titer and erythromycin A concentration in mutant were 46.9 (12740.5 mu g/mL) and 64.9 % (8094.4 mu g/mL) higher than those in original strain, respectively, which were comparable to industrial erythromycin production. The specific glucose and n-propanol consumption rates were increased by 52.4 and 39.8 %, respectively. During the rapid erythromycin synthesis phase, the yield of erythromycin on n-propanol also increased from 24.3 % in control group to 66.9 % in mutant group. Meanwhile, the specific formation rates of methylmalonyl-CoA and propionyl-CoA, two crucial precursors for erythromycin synthesis, were 1.89-and 2.02folds higher in the mutant strain, respectively.