文献类型：期刊文献

英文题名：Rational Design of Artificial Biofilms as Sustainable Supports for Whole-Cell Catalysis Through Integrating Extra- and Intracellular Catalysis

作者：Dong, Hao[1,4];Zhang, Wenxue[1];Zhou, Shengmin[1];Ying, Hanjie[2];Wang, Ping[1,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn 1, Shanghai 200237, Peoples R China;[2]Nanjing Tech Univ, Natl Engn Res Ctr Biotechnol, 30 Puzhu Rd S, Nanjing 211816, Jiangsu, Peoples R China;[3]Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA;[4]Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

年份：2022

卷号：15

期号：17

外文期刊名：CHEMSUSCHEM

收录：;EI(收录号：20222912366054);WOS:【SCI-EXPANDED(收录号:WOS:000823701700001)】；

基金：This work was sponsored by the National Natural Science Foundation of China (21636003, 21672065, and 22077032), the International S&T Innovation Cooperation Key Project (2017YFE0129600), the Fundamental Research Funds for the Central Universities (22221818014), and the 111 Project (B18022).

语种：英文

外文关键词：biofilm catalysts; biotransformations; enzymes; immobilization; rational design

摘要：Biofilms are promising candidates for sustainable bioprocessing applications. This work presents a rational design of biofilm catalysts by integrating extra- and intracellular catalysis systems with optimized substrate channeling to realize efficient multistep biosynthesis. An assembly of four enzymes in a "three-in-one" structure was achieved by rationally placing the enzymes on curli nanofibers, the cell surface, and inside cells. The catalytic efficiency of the biofilm catalysts was over 2.8 folds higher than that of the control whole-cell catalysis when the substrate benzaldehyde was fed at 100 mm. The highest yield of d-phenyllactic acid catalyzed by biofilm catalysts under optimized conditions was 102.19 mm, also much higher than that of the control catalysis test (52.29 mm). The results demonstrate that engineered biofilms are greatly promising in integrating extra- and intracellular catalysis, illustrating great potentials of rational design in constructing biofilm catalysts as sustainable supports for whole-cell catalysis.