文献类型：期刊文献

英文题名：PDL regeneration via cell homing in delayed replantation of avulsed teeth

作者：Zhu, Wenting[1];Zhang, Qian[1];Zhang, Yang[1];Cen, Lian[2,3];Wang, Jun[1]

机构：[1]Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Pediat Dent, Shanghai 200011, Peoples R China;[2]E China Univ Sci & Technol, Sch Chem Engn, Shanghai 200237, Peoples R China;[3]Natl Tissue Engn Ctr China, Shanghai 200241, Peoples R China

年份：2015

卷号：13

外文期刊名：JOURNAL OF TRANSLATIONAL MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000364756200001)】；

基金：This study is financially supported by grants from the Science and Technology Commission of Shanghai (Grant No: 124119b0101) and National "973" Project Foundation of China (Grant No: 2010CB944804). The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.

语种：英文

摘要：Background: This study was aimed to investigate whether regeneration of periodontal ligament (PDL) like tissue could be promoted by stromal cell-derived factor-1 (SDF1) and bone morphogenetic protein-7 (BMP7) induced cell homing in delayed replantation of avulsed teeth. Methods: Canine mandibular premolar teeth were first extracted and air-dried for 2 h followed by complete detachment of their PDL tissues. The crown and pulp of the teeth were also removed. Twenty-four roots divided into two groups (n = 12/group) were used for the following in vivo transplantation. The roots of Group A were treated with 17 % EDTA for 24 h to achieve demineralization, and then coated with SDF1 and BMP7 supplemented collagen solution. The roots of Group B were similarly treated except being coated with a pristine collagen solution. The above roots were transplanted in the sockets that formed previously during tooth extraction. At 6 months' post-operation, PDL-like tissue composed of spindle-shaped cells, capillaries and highly organized collagen fibers was observed in the interstitial space between the avulsed root surface and surrounding alveolar bone in Group A. The neo-fibers inserted deeply and perpendicularly into the cementum and adjacent bone. The periodontium-like characteristics of the neo-tissue was confirmed by immunohistochemical staining for collagen I, fibronectin and osteocalcin. Results: A high incidence of PDL re-establishment as 42 % was achieved for samples of Group A. However, no PDL-like tissue was found but root ankylosis and replacement resorption as well as inflammatory resorption was observed in the replanted roots of Group B. Conclusions: It can be confirmed that avulsed teeth could be successfully rescued even in delayed transplantation to avoid dentoalveolar ankylosis or replacement resorption via the current developed cell homing method.