文献类型：期刊文献

英文题名：Unique Tr-Output Optical Probe for Specific and Ultrasensitive Detection of Hydrazine

作者：Cui, Lei[1];Ji, Chunfei[1];Peng, Zhixing[1];Zhong, Lin[1];Zhou, Chaohui[3];Yan, Luliang[1];Qu, Song[1];Zhang, Shuping[1];Huang, Chusen[3];Qian, Xuhong[2];Xu, Yufang[2]

机构：[1]Shanghai Univ Sci & Technol, Coll Sci, Sch Environm & Architecture, Shanghai 201800, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[3]Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai, Peoples R China

年份：2014

卷号：86

期号：9

起止页码：4611

外文期刊名：ANALYTICAL CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000335719900076)】；

基金：This work is supported by the National Basic Research Program of China (973 Program, 2010CB126100), the National High Technology Research and Development Program of China (863 Program, 2011AA10A207), National Natural Science Foundation of China (21202097, 21236002, and 21302125), the Innovation Fund Project for Graduate Student of Shanghai, and the Fundamental Research Funds for the Central Universities.

语种：英文

摘要：An optical probe based on colorimetric and ratiometric as well as chemiluminometric signal outputs is developed for the specific detection of hydrazine. On the basis of a Gabriel-type reaction, hydrazinolysis of a simple probe CF (4-phtalamide-N-(4'-methylcoumarin) naphthalimide) produces both the fluorescence of 7-amino-4-methylcoumarin with the max emission wavelength changed from 480 to 420 nm (along with a color change from yellow to transparent) and the luminol chemiluminescence activated by H2O2 with a max emission wavelength at 450 nm. The experimental detection limit of hydrazine is 3.2 ppb (0.1 mu M). Selectivity experiments proved CF has excellent selectivity to hydrazine over other interfering substances. Probe CF was also successfully applied in the vapor hydrazine detection over other interfering volatile analytes. Furthermore, the probe CF loaded thin-layer chromatography (TLC) plate for vapor hydrazine detection limit is 5.4 mg/m(3) which is well below the half lethal dose of hydrazine gas for mice (LC50(mice), 330 mg/m(3)) and National Institute of Occupational Safety and Health's immediately dangerous to life or health limit (NIOSHIDLH, 66 mg/m(3)). With H2O2, only hydrazinolysis product luminol can be lighted at 450 nm, other species have no signal. Probe CF can also be used for the detection of hydrazine in HeLa cells.