文献类型：期刊文献

中文题名：Artificial cilia for soft and stable surface covalent immobilization of bone morphogenetic protein-2

作者：Qi Gan[1,2];Lina Chen[1];Ho-Pan Bei[3];Sze-Wing Ng[1];Han Guo[4];Guoqiang Liu[1];Hao Pan[2];Changsheng Liu[2];Xin Zhao[3];Zijian Zheng[1,5]

机构：[1]Laboratory for Advanced Interfacial Materials and Devices,School of Fashion and Textiles,The Hong Kong Polytechnic University,Kowloon,99077,Hong Kong Special Administrative Region of China;[2]Key Laboratory for Ultrafine Materials of Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai,200237,PR China;[3]Department of Biomedical Engineering,The Hong Kong Polytechnic University,Hung Hom,Hong Kong Special Administrative Region of China;[4]Shanghai Synchrotron Radiation Facility,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai,201204,PR China;[5]Department of Applied Biology and Chemical Technology,Faculty of Science,The Hong Kong Polytechnic University,Kowloon,99077,Hong Kong Special Administrative Region of China

年份：2023

期号：6

起止页码：551

中文期刊名：Bioactive Materials

外文期刊名：生物活性材料（英文）

收录：Scopus;CSCD:【CSCD2023_2024】；PubMed;

基金：financial support from RGC Senior Research Fellow Scheme(SRFS2122-5S04),Research Grants Council of Hong Kong;Collaborative Research Fund(C5044-21GF)from the Research Grants Council of Hong Kong and National Excellent Young Scientists Fund(Hong Kong and Macao,82122002),National Natural Science Foundation of China;support from National Natural Science Foundation of China(No.32071337);Shanghai Pujiang Program(20PJ1402600).

语种：英文

中文关键词：Polymer brush;Poly(glycidyl methacrylate);Surface modification;Protein immobilization;Bioactivity

摘要：Preservation of growth factor sensitivity and bioactivity(e.g.,bone morphogenetic protein-2(BMP-2))post-immobilization to tissue engineering scaffolds remains a great challenge.Here,we develop a stable and soft surface modification strategy to address this issue.BMP-2(a model growth factor)is covalently immobilized onto homogeneous poly(glycidyl methacrylate)(PGMA)polymer brushes which are grafted onto substrate surfaces(Au,quartz glass,silica wafer,or common biomaterials)via surface-initiated atom transfer radical polymerization.This surface modification method multiplies the functionalized interfacial area;it is simple,fast,gentle,and has little effect on the loaded protein owing to the cilia motility.The immobilized BMP-2(i-BMP-2)on the surface of homogeneous PGMA polymer brushes exhibits excellent bioactivity(-87%bioactivity of free BMP-2 in vitro and 20%-50%higher than scaffolds with free BMP-2 in vivo),with conformation and secondary structure well-preserved after covalent immobilization and ethanol sterilization.Moreover,the osteogenic activity of i-BMP-2 on the nanoline pattern(PGMA-poly(N-isopropylacrylamide))shows-110%bioactivity of free BMP-2.This is superior compared to conventional protein covalent immobilization strategies in terms of both bioactivity preservation and therapeutic efficacy.PGMA polymer brushes can be used to modify surfaces of different tissue-engineered scaffolds,which facilitates in situ immobilization of growth factors,and accelerates repair of a wide range of tissue types.