文献类型：期刊文献

英文题名：Rational design and synthesis of molecularly imprinted polymers (MIP) for purifying tylosin by seeded precipitation polymerization

作者：Zeng, Hainan[1];Yu, Xue[1];Wan, Junfen[1];Cao, Xuejun[1]

机构：[1]East China Univ Sci & Technol, Dept Bioengn, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2020

卷号：94

起止页码：329

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20202008671744);WOS:【SCI-EXPANDED(收录号:WOS:000537690100036)】；

语种：英文

外文关键词：Molecularly imprinted polymer; Core-shell structure; Molecular simulation; Solid phase extraction; Tylosin; Precipitation polymerization

摘要：A useful approach was proposed to easily synthesize molecularly imprinted adsorbent for the purification of tylosin from broth. Firstly, by molecular simulation based on density functional theory, methacrylic acid was chosen as a functional monomer by comparing the binding energy. Second, a novel method of polymerization based on precipitation polymerization with added seeds was used in water-mixed solvent for the preparation of water-compatible micron-sized MIP. Its static adsorption capacity for tylosin in aqueous solution was estimated to be 106.5 mg/g with the highest imprinting factor (IF) of 3.6. The selectivity coefficient (alpha) of tylosin to desmycosin was 3.3. The antibiotic in fermentation broth could be purified by means of molecularly imprinted solid phase extraction (MISPE), which allows MIP to be used for the purification of tylosin from a complex sample.