文献类型：期刊文献

英文题名：High cell density fermentation of Gluconobacter oxydans DSM 2003 for glycolic acid production

作者：Wei, Guodong[1];Yang, Xuepeng[2];Gan, Tula[1];Zhou, Wenyu[1];Lin, Jinping[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China;[2]Zhengzhou Univ Light Ind, Coll Food & Biol Engn, Zhengzhou 450002, Peoples R China

年份：2009

卷号：36

期号：8

起止页码：1029

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20242616358126);WOS:【SCI-EXPANDED(收录号:WOS:000268301200004)】；

基金：The work was supported by the National Basic Research Program ( 973 program, No. 2009CB724703) and the program Shanghai Subject Chief Scientist ( No. 06XD14006).

语种：英文

外文关键词：Gluconobacter oxydans; Uniform design; Fed-batch; High-density fermentation; Bioconversion; Ethylene glycol; Glycolic acid

摘要：Gluconobacter oxydans has a lower biomass yield. Uniform design (UD) was applied to determine the optimum composition of the critical media and their mutual interactions for increased biomass yield of Gluconobacter oxydans DSM 2003 in shake flasks. Fed-batch fermentation process for biomass was optimized in a 3.7-l fermentor. By undertaking a preliminary and improved fed-batch fermentation-process strategy, a cell density of 6.0 g/l (DCW) was achieved in 22 h and 14.1 g/l (DCW) in 35 h, which is the highest cell density of G. oxydans produced thus far in a 3.7-l bioreactor. The biomass production was increased by 135% compared with that using the original cultivation strategy. Bioconversion of ethylene glycol to glycolic acid was catalyzed by the resting cells of G. oxydans DSM 2003, and conversion rate reached 86.7% in 48 h. In summary, the approach including high-density fermentation of G. oxydans DSM 2003 and bioconversion process was established and proved to be an effective method for glycolic acid production.