文献类型：期刊文献

中文题名：海洋放线菌Y12-26中抗真菌活性代谢产物的分离纯化与结构鉴定

英文题名：Purification and structure elucidation of antifungal bioactive metabolites from marine actinomycete Y12-26

作者：程亮[1];罗明明[1];吴继纲[1];郝有武[1];徐文平[1];陶黎明[1]

机构：[1]华东理工大学上海市化学生物学(芳香杂环)重点实验室,上海200237

年份：2017

卷号：42

期号：8

起止页码：631

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

基金：国家科技支撑项目(No.2011BAE06B04-1)

语种：中文

中文关键词：海洋放线菌;分离纯化;结构鉴定;抗真菌活性

外文关键词：Marine actinomycete; Purification; Structural elucidation; Antifungal bioactivity

摘要：目的研究海洋放线菌Y12-26发酵液中对几种植物病原真菌具有抑制作用的次生代谢活性物质。方法发酵离心上清液用等体积正丁醇萃取,得到发酵液浸膏。浸膏采用正相硅胶柱色谱、制备TLC和半制备HPLC等方法进行分离纯化该菌株活性次级代谢产物,并运用MS、~1H NMR和^(13)C NMR等波谱技术并结合文献比对鉴定所分离的单体化合物。结果从28g正丁醇萃取物中分离纯化并鉴定了3个单体活性化合物,分别为:salicylamide(1)、iturin A-2(2)、daidzein-4',7-di-α-L-rhamnoside(3)。结论海洋放线菌Y12-26能代谢产生具有多种生物活性的化合物。化合物1对Botriytis cinerea和Pyricularia oryzae的最低抑菌浓度(minimal inhibitory concentration,MIC)为125μg/mL,化合物2有强烈的抗真菌活性,其对Rhizoctonia solani的MIC为12.5μg/mL,化合物3对Botriytis cinerea的MIC为125μg/mL。本文首次报道大豆素类化合物3抗植物病原真菌活性。
Objective To study the antifungal constituents of a marine actinomycete Y12-26. Methods The fermentation centrifugal supernatant was extracted with an equal volume of butanol. The extracts was isolated and repeatedly purified by column chromatography on silica gel, preparative TLC arid semi-preparative HPLC. Their structures were elucidated by MS, ^1H-NMR, ^13C-NMR spectral analysis and compared with the data of literature. Results Three compounds were isolated and identified as salicylamide （1）, iturin A-2 （2）, daidzein-4＇,7-di-α-L-rhamnoside （3） from 28g butanol extracts. Conclusion The marine actinomycete Y12-26 can produce the compounds with many biological activities. The MIC of compound 1 against Botrytis cinerea and Pyricularia oryzae is 125μg/mL, compound 2 shows significant antifungal bioactivity against Rhizoctonia solaniis 12.5μg/mL, compound 3 against Botrytis cinereais 125μg/mL. Compound 3 is reported firstly for the inhibitory activities against plant-pathogenic fungus.