文献类型：期刊文献

英文题名：Integrated pathway engineering and transcriptome analysis for improved astaxanthin biosynthesis in Yarrowia lipolytica

作者：Wang, Dan-Ni[1];Feng, Jie[1];Yu, Chen-Xi[1];Zhang, Xin-Kai[1];Chen, Jun[1];Wei, Liu-Jing[1];Liu, Zhijie[2];Ouyang, Liming[1];Zhang, Lixin[1];Hua, Qiang[1,3];Liu, Feng[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Hubei Univ Technol, Minist Educ & Hubei Prov,Minist Educ, Cooperat Innovat Ctr Ind Fermentat, Key Lab Fermentat Engn, Wuhan 430068, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：7

期号：4

起止页码：1133

外文期刊名：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000848099500002)】；

基金：This study was supported by the National Key R & D Program of China (2017YFE0115600, 2019YFA0904302, 2020YFA0907800) ; and the National Natural Science Foundation of China (21576089, 21776081) .

语种：英文

外文关键词：Astaxanthin; Pathway engineering; Transcriptome analysis; Yarrowia lipolytica

摘要：Astaxanthin is a high value carotenoid with a broad range of commercial applications due to its superior antioxidant properties. In this study, 8-carotene-producing Yarrowia lipolytica XK17 constructed in the lab was employed for astaxanthin biosynthesis. The catalytic effects of 8-carotene ketolase CrtW and 8-carotene hydroxylase CrtZ from various species were investigated. The PspCrtW from Paracoccus sp. and HpCrtZ# from Haematococcus pluvialis were confirmed to be the best combination in converting 8-carotene. Several key bottlenecks in biomass and astaxanthin biosynthesis were effectively eliminated by optimizing the expression of the above enzymes and restoring uracil/leucine biosynthesis. In addition, the effects of astaxanthin biosynthesis on cell metabolism were investigated by integrated analysis of pathway modification and transcriptome information. After further optimization, strain DN30 was able to synthesize up to 730.3 mg/L astaxanthin in laboratory 5-L fermenter. This study provides a good metabolic strategy and a sustainable development platform for highvalue carotenoid production.