文献类型：期刊文献

英文题名：PURIFICATION OF HUMAN SERUM-ALBUMIN BY DYE-LIGAND AFFINITY-CHROMATOGRAPHY

作者：LI, ZX; CHEN, KC

机构：[1]E CHINA UNIV CHEM TECHNOL,FINE CHEM RES INST,SHANGHAI 200237,PEOPLES R CHINA

年份：1993

卷号：22

期号：1

起止页码：27

外文期刊名：DYES AND PIGMENTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:A1993LN93700003)】；

语种：英文

摘要：In this paper, four of the eight synthetic reactive dyes studied were used to isolate human serum albumin after these ligands were immobilized to cross-linked agarose gels. UV spectrophotometric methods were used to study the absorbing capacity of the eight dyes. The relation between the adsorbing capacity or specificity of the adsorbents and the concentration of the adsorbents or the pH of the 10 mM phosphate buffers was studied, six different matrices (Xianfen beads, cross-linked dextran, polyvinyl alcohol fibre, silanized silica, cross-linked agarose and agarose beads) were evaluated. The best conditions for isolating human serum albumin were ascertained A larger scale dye-ligand chromatography system was used to purify human serum albumin and the cost factors involved were evaluated.