文献类型：期刊文献

英文题名：Impact of co-deficiency of RpoN and RpoS on stress tolerance, virulence and gene regulation in Edwardsiella tarda

作者：Liu, Enfu[1];Ye, Jiang[1];Song, ShanShan[1];Wang, Keping[1];Zhang, Yuanxing[1];Zhang, Huizhan[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：54

期号：7

起止页码：678

外文期刊名：JOURNAL OF BASIC MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000339667600007)】；

基金：We thank Dr. Zhaolan Mo (Institute of Oceanology, Chinese Academy of Sciences, China) for kindly sending strains and plasmids used for the mutant construction in this study. This work was supported by the National Special Fund for State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Edwardsiella tarda EIB202; rpoN rpoS; Stress tolerance; Virulence; Housekeeping genes

摘要：Edwardsiella tarda the etiological agent for edwardsiellosis, a devastating fish disease prevailing in worldwide aquaculture industries was subjected to a molecular genetic study. To research into the influence when RpoN (sigma(54)) and RpoS (sigma(38)) were deleted simultaneously, the double deletion mutant of RpoN (sigma(54)) and RpoS (sigma(38)), namely rnrs, was constructed. Firstly, RpoN and RpoS are both essential for H2O2, starvation, high osmotic pressure and acid resistance, which have synergistic effect. Secondly, virulence of rnrs reduces significantly compared to E. tarda EIB 202 WT, Delta rpoN mutant and Delta rpoS mutant. Furthermore, transcriptional control of rpoS by rpoN in stationary phase was observed through qRT-PCR, while rpoS had no influence on rpoN in the level of transcription. Meanwhile, regulation of flagellar sigma factor sigma(F) (FliA) and other flagella-related genes including flgA, flgK, flgL, motA, and motB by rpoS, and rpoN was found. fliA and other flagella-related genes were controlled positively by rpoN, while negatively by rpoS. At last, two differential expression genes in transcriptional level of rnrs strain were detected by DD-RT-PCR, namely cheY and narK. This study therefore indicated interaction between sigma factors RpoN and RpoS, which modulates stress response, virulence, motility, and provides new insights into the regulatory networks of E. tarda.