文献类型：期刊文献

英文题名：Establishment of a Fingerprint of Raspberries by LC

作者：Chen, Juan[1];Lu, Yan-Hua[1];Wei, Dong-Zhi[1];Zhou, Xiao-Li[2]

机构：[1]E China Univ Chem Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Inst Technol, Speciry Fac, Shanghai 200235, Peoples R China

年份：2009

卷号：70

期号：5-6

起止页码：981

外文期刊名：CHROMATOGRAPHIA

收录：;EI(收录号：20242616412606);WOS:【SCI-EXPANDED(收录号:WOS:000269879000043)】；

基金：This work was supported by the Shanghai Leading Academic Discipline Project (B505) and by the National Special Fund for State Key Laboratory of Bioreactor Engineering, grant no. 2060204.

语种：英文

外文关键词：Column liquid chromatography; Raspberries; Hyperoside; Tiliroside; Fingerprint; Quality control

摘要：To monitor the quality of raspberries, a simple, practicable, and feasible method, high-performance liquid chromatography coupled with photodiode-array detection, has been developed both for fingerprinting analysis and for quantitative analysis of hyperoside and tiliroside. Compounds were separated on a 4.6 mm x 250 mm, 5-mu m particle, C-18 column with a mobile phase gradient prepared from acetonitrile and 0.5% (v/v) aqueous glacial acetic acid. The mobile phase flow rate was 1.0 mL min(-1), the temperature 30 A degrees C, the injection volume 10 mu L, and the detection wavelength 340 nm. Under the optimum conditions the two compounds could be well separated with good linear relationships between response and amount in the range 0.024-2.380 mu g for hyperoside and 0.026-2.570 mu g for tiliroside. Recovery of hyperoside and tiliroside was 99.3 and 106.0%, respectively. The standardized chromatographic fingerprint and the similarity of samples were calculated by use of software. Principal-components analysis was used to differentiate and classify 14 samples on the basis of the area of seven common peaks. Combination of chemical fingerprinting and the quantitative method with principal-component analysis may provide a firm basis for further research on quality control of raspberries.