文献类型：期刊文献

中文题名：Production of Human Epidermal Growth Factor in Fed-batch Culture of Acetate-tolerant Escherichia coli

英文题名：Production of Human Epidermal Growth Factor in Fed-batch Culture of Acetate-tolerant Escherichia coli

作者：韩云[1];李志敏[1];杜鹏[2];甘人宝[2];叶勤[1]

机构：[1]State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai ,200237, China;[2]Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China

年份：2007

卷号：15

期号：5

起止页码：760

中文期刊名：Chinese Journal of Chemical Engineering

外文期刊名：中国化学工程学报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2011_2012】；

基金：the National High Technology Research and Development Program of China(863 Program,2002AA217021).

语种：中文

中文关键词：human epidermal growth factor; acetate-tolerance; Escherichia coli; pH

外文关键词：补料培养;分批培养;耐乙酸大肠杆菌;人表皮生长因子

摘要：An acetate-tolerant mutant of Escherichia coli DH5α, DA19, was used for secretory production of human epidermal growth factor （hEGF） whose expression was under the control of phoA promoter. The recombinant cells were cultured in a chemically defined medium, and glucose was added at different specific provision rates during the growth and expression phases. It was found that pH had a significant effect on the extracellular hEGF production. The extracellular hEGF concentration was 75.5mg·L^-1, 5.2-fold of the level reached at pH 7.0, even though more acetate was produced. Nitrogen source was limited in the later growth phase. Supplementation of ammonium promoted the consumption of phosphate and reduced the time to exhaust phosphate, but the extracellular hEGF production was similar to that without supplementation of ammonium.
Escherichia 关口 i DH5 的醋酸盐容忍的异种， DA19，被用于其表情在 phoA 的控制下面的人的表皮的生长因素(hEGF ) 的能分泌的生产倡导者。recombinant 细胞在化学上定义的媒介是有教养的，并且葡萄糖在生长和表示阶段期间以不同特定的预备率被增加。pH 在细胞外的 hEGF 生产上有重要效果，这被发现。细胞外的 hEGF 集中是 75.5mg .L ? 1，在 pH 到达的水平的 5.2 褶层 7.0，尽管更多的醋酸盐被生产。氮来源在以后的生长阶段被限制。铵的补充支持了磷酸盐的消费并且减少了时间用尽磷酸盐，而是细胞外的 hEGF 生产没有铵的补充，类似于那。