文献类型：期刊文献

英文题名：A BMP-2-triggered in vivo osteo-organoid for cell therapy

作者：Dai, Kai[1,2,3];Zhang, Qinghao[1,2];Deng, Shunshu[1,2];Yu, Yuanman[1,2];Zhu, Fuwei[1,2];Zhang, Shuang[1,2];Pan, YuanZhong[1,2];Long, Dandan[1,2];Wang, Jing[1,3,4];Liu, Changsheng[2,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Biomed Mat, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China

年份：2023

卷号：9

期号：1

外文期刊名：SCIENCE ADVANCES

收录：;EI(收录号：20230413418086);WOS:【SCI-EXPANDED(收录号:WOS:000937027000025)】；

基金：This research was supported by the National Natural Science Foundation of China for Innovative Research Groups (no. 51621002), the National Key R & D Program of China (no. 2018YFE0201500), the National Natural Science Foundation of China (no. 31870953), the Foundation of Frontiers Science Center for Materiobiology and Dynamic Chemistry (no. JKVD1211002), Basic Science Center Program (no. T2288102), the Key Program of the National Natural Science Foundation of China (no. 32230059), the Wego Project of Chinese Academy of Sciences [no. (2020) 005], and the Project of National Facility for Translational Medicine (Shanghai) (TMSK-2021-134).

语种：英文

外文关键词：Bone - Cell culture - Flowcharting - Mammals - Proteins - Scaffolds (biology)

摘要：Cell therapies and regenerative medicine interventions require an adequate source of therapeutic cells. Here, we demonstrate that constructing in vivo osteoorganoids by implanting bone morphogenetic protein-2-loaded scaffolds into the internal muscle pocket near the femur of mice supports the growth and subsequent harvest of therapeutically useful cells including hematopoietic stem/progenitor cells (HSPCs), mesenchymal stem cells (MSCs), lymphocytes, and myeloid cells. Profiling of the in vivo osteo-organoid maturation process delineated three stages-fibroproliferation, osteochondral differentiation, and marrow generation-each of which entailed obvious changes in the organoid structure and cell type distribution. The MSCs harvested from the osteochondral differentiation stage mitigated carbon tetrachloride (CCl4)-induced chronic liver fibrosis in mice, while HSPCs and immune cells harvested during the marrow generation stage rapidly and effectively reconstituted the impaired peripheral and solid immune organs of irradiated mice. These findings demonstrate the therapeu-tic potentials of in vivo osteo-organoid-derived cells in cell therapies.