文献类型：期刊文献

英文题名：Discrimination of biothiols in different media with NBD-F as the probe

作者：Song, Lun[1];Ma, Li-Min[1];Sun, Qian[1];Zhang, Wei-Bing[1];Lan, Min-Bo[1];Qian, Jun-Hong[1,2]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2016

卷号：27

期号：3

起止页码：330

外文期刊名：CHINESE CHEMICAL LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000374799400006)】；

基金：This work was financially supported by Shanghai Municipal Natural Science Foundation (No. 15ZR1409000) and the open fund of Shanghai Key Laboratory of Chemical Biology (No. SKLCB-2013-03).

语种：英文

外文关键词：NBD-F; Biothiols; Surfactant micelles; Fluorescent probe

摘要：4-Fluoro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-F) was employed as a colorimetric probe for differential detection of biothiols in different media. The spectral response and the selectivity of NBD-F toward thiols were significantly improved by surfactant micelles. Mercapto group exhibited high reactivity in all the solvents (including Tris-HCl buffer solution, CTAB and SDS micelles). The 4-thioether derivatives of NBD-F reacting with Cys and Hcy but not GSH could transfer to the corresponding 4-amino-substituents via intramolecular nucleophilic aromatic substitution, thus, GSH could be discriminated from Cys/Hcy. In CTAB micelles, the reaction product of NBD-F with Cys is non-fluorescent and it absorbs in long wavelength region. According to the spectral responses of NBD-F toward different low-molecular weight thiols, we could identify Cys, Hcy and GSH from each other. (C) 2015 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.