文献类型：期刊文献

英文题名：Highly Efficient Multiple-Labeling Probes for the Visualization of Enzyme Activities

作者：Song, Heng[1];Li, Yuyao[1];Chen, Yefeng[1];Xue, Chenghong[1];Xie, Hexin[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：25

期号：61

起止页码：13994

外文期刊名：CHEMISTRY-A EUROPEAN JOURNAL

收录：;EI(收录号：20194407598134);WOS:【SCI-EXPANDED(收录号:WOS:000489316700001)】；

基金：Financial support from The Recruitment Program for Young Professionals, NSFC-BRICS (81861148020), the Research Program of State Key Laboratory of Bioreactor Engineering, and the Fundamental Research Funds for the Central Universities are gratefully acknowledged.

语种：英文

外文关键词：alkaline phosphatase; enzyme activity; imaging; immobilization; quinone methide

摘要：Quinone methide (QM) as a latent trapping unit has been widely explored in activity-based self-immobilizing reagents. However, further application of this strategy has been largely hampered by the limited labeling efficiency to proteins. In this study, a thorough investigation on the labeling efficiency and the structure of QM-based trapping unit is presented, from which a QM with multiple leaving groups was identified as an optimal trapping unit. An alkaline phosphatase (ALP) immobilizing reagent featured with this multiple-labeling trapping unit exhibited lower nonspecific binding and, remarkably, a significantly higher labeling efficiency over other immobilizing reagents upon enzymatic activation. The utility of this imaging reagent was further demonstrated with the in vitro and in vivo visualization of the ALP activities. Furthermore, the multiple functional trapping unit may find greater value in the other activity-based immobilizing probes.