文献类型：期刊文献

英文题名：Ultraviolet and tandem mass spectrometry for simultaneous quantification of 21 pivotal metabolites in plasma from patients with diabetic nephropathy

作者：Xia, Jian-Fei[2];Liang, Qiong-Lin[1];Liang, Xiao-Ping[2];Wang, Yi-Ming[1];Hu, Ping[3];Li, Ping[4];Luo, Guo-An[1,2]

机构：[1]Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharmaceut, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Coll Chem & Mol Engn, Shanghai 200237, Peoples R China;[4]Sinojapanese Friendship Hosp, Clin Med Res Inst, Beijing, Peoples R China

年份：2009

卷号：877

期号：20-21

起止页码：1930

外文期刊名：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

收录：;EI(收录号：20092512141100);WOS:【SCI-EXPANDED(收录号:WOS:000267453800020)】；

基金：The authors acknowledge the supports of Sino-Japanese Friendship Hospital (Beijing, China) who offered samples. The studies have been supported by grant from the National Basic Research Development Program of China (973 Program, No. 2005CB523503), and from the National Natural Science Foundation of China (No. 90709045).

语种：英文

外文关键词：Diabetic nephropathy; Purine and pyrimidine metabolism; Ultraviolet and tandem mass spectrometry; Biomarker

摘要：A sensitive and specific method was developed for simultaneous determination of 21 compounds related to the diabetic nephropathy (DN) in a single analysis using high-performance liquid chromatography coupled to ultraviolet and tandem mass spectrometry (HPLC-UV/MS/MS) in human plasma. With retention times and MS/MS for peak identification, both UV and MS detectors were used for quantification. Calibration curves suitable for the analysis of plasma were linear (r(2) > 0.998) with limits of detection (LOD) from 10 to 1000 ng/mL. Intraday relative standard deviation (R.S.D.) and interday R.S.D. were both lower than 15%. With the case and control study, we found five potential biomarkers of DN, including adenosine, inosine, uric acid, xanthine and creatinine. (C) 2009 Elsevier B.V. All rights reserved.