文献类型：期刊文献

英文题名：Microbial production of 2,3-butanediol by a surfactant (serrawettin)-deficient mutant of Serratia marcescens H30

作者：Zhang, Liaoyuan[1];Sun, Jian'an[1];Hao, Yingli[1];Zhu, Jiawen[2];Chu, Ju[1];Wei, Dongzhi[1];Shen, Yaling[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Chem Engn Res Ctr, Shanghai 200237, Peoples R China

年份：2010

卷号：37

期号：8

起止页码：857

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20242716586222);WOS:【SCI-EXPANDED(收录号:WOS:000280845200011)】；

基金：This work was supported by the Chinese National Program for High Technology and Development (863 Program) (No. 2006AA02Z243), Shanghai Leading Academic Discipline Project (Project B505), and State Key Laboratory special fund (No. 2060204).

语种：英文

外文关键词：2,3-Butanediol; Serrawettin W1 synthase; Inactivation; Serratia marcescens; Fed-batch

摘要：Serrawettin W1 produced by Serratia marcescens is a surface-active exolipid resulting in a lot foam formation during the 2,3-butanediol (2,3-BD) fermentation process. In order to avoid excessive addition of antifoam agent and microbial contamination, S. marcescens mutants deficient in serrawettin W1 formation were successfully constructed through insertional inactivation of the swrW gene coding for serrawettin W1 synthase. The shake flask and batch experiments suggested that disruption of the swrW gene led to significant reduction of the foam formation and improved 2,3-BD production a little. Ultimately, fed-batch culturing of the mutant afforded a maximum 2,3-BD concentration of 152 g l(-1) with a productivity of 2.67 g l(-1) h(-1) and a yield of 92.6% at 57 h.