文献类型：期刊文献

中文题名：微流控剪切条件下的聚肽自组装行为

英文题名：Self-assembly Behavior of Polypeptides under Microfluidic Shear Condition

作者：李芊晴[1];高梁[1];林嘉平[1];蔡春华[1]

机构：[1]华东理工大学材料科学与工程学院,上海200237

年份：2025

卷号：56

期号：11

起止页码：2033

中文期刊名：高分子学报

外文期刊名：Acta Polymerica Sinica

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001629497000013)】；北大核心:【北大核心2023】；

语种：中文

中文关键词：微流控;剪切;聚肽自组装;扭曲状聚集体

外文关键词：Microfluidic;Shear;Polypeptide self-assembly;Twisted aggregates

摘要：微流控通道中独特的剪切环境为构筑复杂纳米结构带来了新的机遇.本研究设计了一种环型微流控通道,利用剪切作用使刚性聚肽形成与溶液自组装不同的扭曲状聚集体.通过调控剪切速率和含水量,结合圆二色谱(CD)与同步辐射广角X射线衍射测试(WAXS),探究了聚(γ-苄基-L-谷氨酸酯)(PBLG)形成扭曲状聚集体的机理.在选择性溶剂(水)的剪切作用下,聚肽链间距减小、链有序排列更紧密,同时其α-螺旋构象向PPⅡ-螺旋构象转变,这使聚集体产生内扭力,因而在剪切条件下发生形貌转变,形成新颖的扭曲状形貌结构.研究结果为定向设计多级结构提供了新策略.
The unique shear environment in microfluidic channels offers new opportunities for constructing complex nanostructures.In this study,a ring-shaped microfluidic channel was designed to utilize shear forces to induce rigid polypeptides for forming twisted aggregates that are different from those formed by solution selfassembly.By examining the effects of shear rate and water content,and combining circular dichroism(CD)and synchrotron wide-angle X-ray scattering(WAXS)tests,the formation mechanism of twisted aggregate of poly(γ-benzyl-L-glutamate)(PBLG)was revealed.Under the shear of a selective solvent(water),the distance between polypeptide chains decreases,and the chain arrangement becomes more ordered and compact.Meanwhile,theα-helix conformation of the polypeptide transforms into PPⅡ-helix conformation,which generates an internal twisted force in the aggregates,forming a novel twisted aggregate morphology.The research results can provide a new strategy for the directed design of hierarchical structures.