文献类型：期刊文献

英文题名：Protein Immobilization onto Cationic Spherical Polyelectrolyte Brushes Studied by Small Angle X-ray Scattering

作者：Wang, Weihua[1,2];Li, Li[1];Henzler, Katja[3];Lu, Yan[2,4];Wang, Junyou[1];Han, Haoya[1];Tian, Yuchuan[1];Wang, Yunwei[1];Zhou, Zhiming[1];Lotze, Gudrun[5];Narayanan, Theyencheri[5];Ballauff, Matthias[2,4];Guo, Xuhong[1,6]

机构：[1]East China Univ Sci & Technol, State Key Lab Chem Engn, Shanghai 200237, Peoples R China;[2]Helmholtz Zentrum Berlin Mat & Energie GmbH, Soft Matter & Funct Mat, Hahn Meitner Pl 1, D-14109 Berlin, Germany;[3]Paul Scherer Inst, Lab Synchrotron Radiat & Femtochem, CH-5232 Villigen, Switzerland;[4]Humboldt Univ, Inst Phzs, D-12489 Berlin, Germany;[5]European Synchrotron Radiat Facil, F-38043 Grenoble, France;[6]Shihezi Univ, Engn Res Ctr Mat Chem Engn Xinjiang Bingtuan, Xinjiang 832000, Peoples R China

年份：2017

卷号：18

期号：5

起止页码：1574

外文期刊名：BIOMACROMOLECULES

收录：;EI(收录号：20172003661003);WOS:【SCI-EXPANDED(收录号:WOS:000401044400014)】；

基金：The financial support by the NSFC Grants (51273063 and 21476143), the Fundamental Research Funds for the Central Universities, 111 Project Grant (B08021), European Synchrotron Radiation Facility, Shanghai Synchrotron Radiation Facility, and the China Scholarship Council are gratefully acknowledged.

语种：英文

外文关键词：Body fluids - Polyelectrolytes - Proteins

摘要：The immobilization of bovine serum albumins (BSA) onto cationic spherical polyelectrolyte brushes (SPB) consisting of a solid polystyrene (PS) core and a densely grafted poly(2-aminoethyl methacrylate hydrochloride) (PAEMH) shell was studied by small-angle X-ray scattering (SAXS). The observed dynamics of adsorption of BSA onto SPB by time-resolved SAXS can be divided into two stages. In the first stage (tens of milliseconds), the added proteins as inbetween bridge instantaneously caused the aggregation of SPB. Then BSA penetrated into the brush layer driven by electrostatic attractions, and reached equilibrium in the second stage (tens of seconds). The amount of BSA immobilized onto brush layer reached the maximum when pH was increased to about 6.1 and BSA concentration to 10 g/L. The cationic SPB were confirmed to provide stronger adsorption capacity for BSA compared to anionic ones.