文献类型：期刊文献

英文题名：Ganoderic acid Mf and S induce mitochondria mediated apoptosis in human cervical carcinoma HeLa cells

作者：Liu, Ru-Ming[2];Zhong, Jian-Jiang[1,2]

机构：[1]Shanghai Jiao Tong Univ, Key Lab Microbial Metab, Minist Educ, Sch Life Sci & Biotechnol, Shanghai 200240, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Bioengn, Shanghai 200237, Peoples R China

年份：2011

卷号：18

期号：5

起止页码：349

外文期刊名：PHYTOMEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000290073100003)】；

基金：Financial support from the Shanghai Science & Technology Commission (project Nos 054319933 and 08DZ1971900) and the Shanghai Leading Academic Discipline Project (project Nos. B203 and B505) is gratefully acknowledged. The authors also thank Ying-Bo Li, Jia-Le Wang and other group members for their assistance during the preparation of ganoderic acids.

语种：英文

外文关键词：Ganoderma lucidum; Ganoderic acid Mf (GA-Mf); Ganoderic acid S (GA-S); Mitochondria; HeLa cells; Apoptosis

摘要：In this work, the effects of a pair of positional isomer of ganoderic acids (GAS), namely ganoderic acid Mf (GA-Mf) and ganoderic acid S (GA-S) purified from the fermented mycelia of Ganoderma lucidum, on induction of cell apoptosis and the apoptotic pathway in HeLa cells were investigated. The results demonstrate that both isomers decreased cell population growth on various human carcinoma cell lines by MTT assay, while GA-Mf had better selectivity between normal and cancer cells. The flow cytometry analysis indicated that treatment of HeLa cells with GA-S caused cell cycle arrest in the S phase, while GA-Mf caused cell cycle arrest in the G1 phase. Compared with GA-S. GA-Mf had more potent increase in the number of early and late apoptotic cells. Treatment of HeLa cells with each isomer decreased the mitochondria membrane potential and caused the release of cytochrome c from mitochondria into the cytosol. In addition, stimulation of caspase-3 and caspase-9 activity was observed. The Bax/Bcl-2 ratio was also increased in GA-treated HeLa cells. The results demonstrated that both isomers GA-Mf and GAS induced apoptosis of human HeLa cells through a mitochondria mediated pathway, but they had the different cell cycle arrest specificity. The findings will be helpful to the development of useful cancer chemopreventive compounds from G. lucidum. (C) 2010 Elsevier GmbH. All rights reserved.