文献类型：期刊文献

英文题名：EFFECTS OF PEPTONE ON HYBRIDOMA GROWTH AND MONOCLONAL-ANTIBODY FORMATION

作者：ZHANG, YX; ZHOU, Y; YU, JT

年份：1994

卷号：16

期号：3

起止页码：147

外文期刊名：CYTOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:A1994QQ56600002)】；

语种：英文

外文关键词：HYBRIDOMA; PEPTONE; MONOCLONAL ANTIBODY; CELL CULTURE

摘要：Hybridoma WuT3 secreting a monoclonal antibody against T lymphocytes was grown in RPMI 1640 medium supplemented with 1% human serum. The effect of the concentration of peptone, as an additive, was investigated on cell growth, monoclonal antibody formation, and cell metabolism over 0-10 g l(-1) range. It was found that 1-5 g l(-1) peptone can significantly promote the growth of cells and increase the formation of monoclonal antibody, especially at 3-5 g l(-1), when both the accumulating level and secretion rate of monclonal antibody are higher than that at other peptone concentrations. Based on glucose, lactate and ammonia analysis data, the efficiency of glycolysis was assessed and the utilization of amino acids was more efficient at 3-5 g l(-1) peptone. The cell growth and monoclonal antibody formation were inhibited at higher peptone concentrations, e.g. 10 g l(-1).