文献类型：期刊文献

英文题名：Monitoring NAD(H) and NADP(H) dynamics during organismal development with genetically encoded fluorescent biosensors

作者：Li, Ting[1,2];Zou, Yejun[1,2];Liu, Shuning[1,2];Yang, Yi[1];Zhang, Zhuo[1,2,3];Zhao, Yuzheng[1,2,3]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[3]Chinese Acad Med Sci, Res Unit New Tech Live Cell Metab Imaging, Beijing, Peoples R China

年份：2022

卷号：11

期号：1

外文期刊名：CELL REGENERATION

收录：WOS:【ESCI(收录号:WOS:001110840500001)】；

基金：This research is supported by National Key Research and Development Program of China (2019YFA0904800 to Y.Zhao), NSFC (32030065, 92049304, 32121005 to Y. Zhao, 31901033 to T.L.), Research Unit of New Techniques for Live-cell Metabolic Imaging (Chinese Academy of Medical Sciences, 2019RU01, 2019-I2M-5-013 to Y.Zhao), Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Y.Zhao), Innovative research team of high-level local universities in Shanghai, the Shanghai Science and Technology Commission (19YF1411300 to T.L.), China Postdoctoral Science Foundation (2019 M651413 to T.L.), the State Key Laboratory of Bioreactor Engineering, the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Cell metabolism; NAD(H) and NADP(H); Genetically encoded fluorescent sensors; Real-time monitoring; Organismal development

摘要：Cell metabolism plays vital roles in organismal development, but it has been much less studied than transcriptional and epigenetic control of developmental programs. The difficulty might be largely attributed to the lack of in situ metabolite assays. Genetically encoded fluorescent sensors are powerful tools for noninvasive metabolic monitoring in living cells and in vivo by highly spatiotemporal visualization. Among all living organisms, the NAD(H) and NADP(H) pools are essential for maintaining redox homeostasis and for modulating cellular metabolism. Here, we introduce NAD(H) and NADP(H) biosensors, present example assays in developing organisms, and describe promising prospects for how sensors contribute to developmental biology research.