文献类型：期刊文献

英文题名：Development of an Acetaldehyde-Specific Fluorescent Probe: Strategy and Applications

作者：Wang, Xicheng[1];You, Haolan[1];Yang, Yi[1];Sheng, Boyan[1];Dou, Hongchao[1];Huang, Junyang[1];Li, Wei[1];Liu, Wenwen[2,3];Xu, Yixiang[1];Li, Jian[1,2,3,4];Shi, Donglei[2,3];Li, Xiaokang[1]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Pharm,Shanghai Key Lab New Drug Design,State K, Shanghai 200237, Peoples R China;[2]Hainan Univ, Sch Pharmaceut Sci, Key Lab Trop Biol Resources Minist Educ, Haikou 570228, Peoples R China;[3]Hainan Univ, Hainan Engn Res Ctr Drug Screening & Evaluat, Sch Pharmaceut Sci, Haikou 570228, Peoples R China;[4]Shihezi Univ, Minist Educ, Sch Pharm, Key Lab Xinjiang Phytomed Resource & Utilizat, Shihezi 832003, Peoples R China

年份：2026

外文期刊名：ACS SENSORS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001853650600001)】；

基金：We gratefully appreciate the financial support from the National Key Research and Development Program of China (grant 2023YFA1802004 to X.L.), the National Natural Science Foundation of China (grant 32121005 to J.L., 82173689 to X.L., and 22407038 to D.S.), the Natural Science Foundation of Shanghai (grant 23ZR1415700 to X.L.), the Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (grant 2021 Sci & Tech 03-28 to X.L.), and the Innovation Program of Shanghai Municipal Education Commission (grant 2021-01-07-00-02-E00104 to J.L.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：acetaldehyde; selective probe; sensing system; ethanol metabolism; fluorescence imaging

摘要：Acetaldehyde (AA), the principal metabolite of ethanol, is a highly reactive species capable of forming covalent adducts with DNA and proteins, contributing to the pathogenesis and progression of multiple diseases. Conventional methods for detecting endogenous AA are commonly invasive and lack specificity against other aldehydes, hampering in-depth investigation of its in vivo mechanisms and biological functions. To overcome these limitations, we developed a fluorescent probe, TZ11008, for the selective and noninvasive detection of AA in living systems. The sensing strategy employs 2-aminobenzaldehyde as an AA-specific "capture"-type recognition unit, which undergoes a chemoselective quinoline cyclization reaction with AA. This reaction quenches the photoinduced electron transfer (PET) effect, resulting in a turn-on fluorescence response that effectively discriminates AA from formaldehyde and other aldehydes. TZ11008 was successfully applied for visual monitoring of AA in live mammalian cells, and zebrafish. Furthermore, utilizing TZ11008, we established an analysis method to monitor dynamic changes in AA levels during ethanol metabolism and evaluated the modulatory effects of aldehyde dehydrogenase 2 (ALDH2) activator and inhibitor. This work provides a "capture"-based strategy for the specific identification of AA in biological systems and demonstrates its utility for preclinical evaluation of interventions targeting alcohol metabolism.